雌激素处理DCs在诱导小鼠同种皮肤移植免疫耐受中的作用  

Roles of dendritic cells treated with 17β-estradiol in immune tolerance induction in skin allograft

作　　者：沈媛[1] 曾耀英[2] 肇静娴[2] 

机构地区：[1]暨南大学附属第一医院,广东广州510632 [2]暨南大学组织移植与免疫实验室,广东广州510632

出　　处：《中国病理生理杂志》2008年第10期2020-2024,共5页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展规划"973"资助项目(No.G1999054303);国家自然科学基金重点资助项目(No.30230350);广东省"十五"重大科技专项资助项目(No.A302020204)

摘　　要：目的:研究雌二醇(17β-estradiol,E2)处理的供体骨髓来源树突细胞(dendritic cells,DCs)在诱导小鼠同种异基因皮肤移植免疫耐受中的作用。方法:体外培养小鼠骨髓来源DCs,然后用E2处理。受体小鼠在皮肤移植前经尾静脉分别输注经E2处理的供体小鼠DCs、供体小鼠成熟DCs以及不成熟DCs,输注PBS为对照组。1周后对受体小鼠进行同种异基因皮肤移植,手术后观察皮肤存活情况,应用流式细胞术检测手术前后受体小鼠外周血中CD4+CD25+调节性T细胞百分率的变化。结果:在同种异基因皮肤移植中,受体小鼠尾静脉输注经E2处理的DCs组与对照组和不成熟DCs组比较,移植皮肤存活时间显著延长,较不成熟DCs组存活时间平均延长10.6d(P<0.01);与对照组和不成熟DCs组比较,E2处理组外周血中CD4+CD25+调节性T细胞百分率明显升高(P<0.01)。结论:在同种异基因小鼠皮肤移植模型中,E2处理的供体小鼠DCs可以延长移植皮肤存活时间。AIM： To study the roles of bone marrow - derived dendritic cells from donor mouse treated with 17β- estradiol （E2 ） in immune tolerance induction in skin allograft. METHODS： Bone marrow- derived dendritic cells from C57 mouse as donor were cultured respectively treated with E2 （ E2 group）. BALB/c mouse as recipient received respectively one injection of dendritic ceils of E2 group, mature dendritic cell group and immature dendritic cell group intravenously. Skin transplantation was performed in the absence of immunosupression after 7 d. Mice that received PBS were served as control. The time of skin survival was observed after transplantation. Flow cytometry was used to analyze the percentage of CD4^＋ CD25^＋ T cells in peripheral blood respectively before and after transplantation. RESULTS： Compared with immature dendritic cells and control group, the time of skin survival in E2 group was significandy longer （ P 〈 0. 01 ）, especially, the time of skin survival still prolonged 10. 6 d after skin rejection in immature dendritic group. The percentage of CD4 ^＋CD25 ^＋regulatory T cells in E2 group was significantly higher than that in immature dendritic cell group and control group （P 〈0. 01 ）. CONCLUSION： In skin allograft model, dendritic cells treated with E2 prolong the allograft survival time.

关 键 词：树突细胞 雌二醇 免疫耐受 皮肤移植 小鼠 

分 类 号：R363[医药卫生—病理学]