黄芪多糖对THP-1巨噬细胞源性泡沫细胞胆固醇流出的影响  

Effect of astragalus polysaccharides on cholesterol efflux in THP-1 macrophage-derived foam cells

作　　者：杨志红[1] 龚伟[1] 陈凤玲[2] 周闻白[1] 张烁[1] 李连喜[1] 梁文昌[1] 杨叶虹[1] 胡仁明[1] 

机构地区：[1]复旦大学附属华山医院内分泌科,上海200040 [2]复旦大学内分泌糖尿病研究所,上海200040

出　　处：《中国病理生理杂志》2008年第10期2029-2032,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30670999)

摘　　要：目的:以THP-1巨噬细胞源性泡沫细胞为研究对象,观察不同浓度黄芪多糖对胆固醇流出和ABCA1基因表达的影响。方法:将THP-1细胞诱导分化成泡沫细胞,用不同浓度黄芪多糖对其干预24h,γ计数仪检测胆固醇流出,RT-PCR及流式细胞仪检测ABCA1的表达。结果:黄芪多糖呈剂量依赖性(10-100mg/L)促进胆固醇流出;增加ABCA1的表达。结论:黄芪多糖可能通过增加ABCA1的表达而促进胆固醇流出。AIM： To study the effect of astragalus polysaccharides （Aps） on cholesterol efflux in THP - 1 macrophage - derived foam cells. METHODS ： After exposed to Aps at different doses, cholesterol efflux and ABCA1 protein levels in cultured THP - 1 macrophage - derived foam cells were determined by a γ counter and flow cytometry, respectively. RESULTS ： Aps increased cholesterol efflux in THP - 1 macrophage - derived foam cells with dose dependent pattern and resulted in an increase in the expression of ABCA1 protein in THP - 1 macrophage - derived foam cells. CONCLUSION ： The increase in cholesterol efflux by Aps might be related to the up - regulation of ABCA1.

关 键 词：黄芪多糖 巨噬细胞 胆固醇 ATP结合匣式转运子 

分 类 号：R363[医药卫生—病理学]