黏着斑激酶相关非激酶对CFSC细胞基质金属蛋白酶-2及其抑制因子mRNA表达的影响  

The effects of FRNK on expressions of MMP-2 mRNA and TIMP-2 mRNA in hepatic stellate cells

作　　者：魏娟[1] 张晓岚[1] 姚冬梅[1] 霍晓霞[1] 申建刚[1] 敦志娜[1] 

机构地区：[1]河北医科大学第二医院消化科,石家庄050000

出　　处：《中华肝脏病杂志》2008年第10期757-761,共5页Chinese Journal of Hepatology

基　　金：河北省自然科学基金（C2008001133）;河北省卫生厅科研课题（2003055）

摘　　要：目的探讨黏着斑激酶相关非激酶（FRNK）对纤维连接蛋白刺激的肝星状细胞CFSC胶原代谢的影响及其分子机制。方法用体外细胞培养技术、脂质体介导法进行FRNK质粒瞬时转染；采用Western blot方法测定FRNK蛋白表达，鉴定转染效果；用^3H Pro掺入技术测定CFSCI型胶原的合成；用RTPCR方法测定FRNK转染前后基质金属蛋白酶2（MMP-2）及其抑制因子（TIMP2）基因在CFSC中表达的变化情况。结果FRNK质粒成功转染CFSC，Ⅰ型胶原合成下降；MMP2基因表达上升，TIMP2基因表达下降，MMP2／TIMP2比值明显上升。结论外源性FRNK在CFSC内大量表达后，CFSC胶原表达减少；FRNK可能通过调节MMP2／TIMP2比值来促进CFSC的胶原降解。Objective To investigate the effects of FAK-related non-kinase （FRNK） on expressions of type I collagen and matrix metalloproteinase-2 （MMP-2） mRNA and tissue inhibitor of metalloproteinase- 2 （TIMP-2） mRNA in rat hepatic stellate cells （HSC）. Methods Using in vitro cell culture technique, FRNK plasmids were transfected into HSC mediated by cationic liposome. Type I collagen synthesis capa- bility in HSC was examined by 3H-Pro incorporation assay. The levels of FRNK in HSC were assayed by Western blot, and the expressions of MMP-2 and TIMP-2 were assayed by RT-PCR on mRNA levels. Results The exposure of HSC to FRNK caused the expression of FRNK protein to be up-regulated, and the FRNK protein contents reached the highest point at 48 h after the transfeetion, P 〈 0.05. The expres- sions of MMP-2 mRNA were up-regulated by FRNK; the expressions of TIMP-2 mRNA were down-regu- lated by FRNK; the ratios of MMP-2 mRNA/TIMP-2 mRNA were enhanced by FRNK. Conclusion After FRNK was transfected, the capability of type I collagen synthesis in HSC was inhibited, which may be related to the up-regulation of MMP-2 mRNA/TIMP-2 mRNA.

关 键 词：肝星状细胞 胶原Ⅰ型 明胶酶A 金属蛋白酶组织抑制剂2 黏着斑激酶相关非激酶 

分 类 号：R575.2[医药卫生—消化系统]