成都地区汉族人群9个STR基因座遗传多态性的研究  

Genetic polymorphism of nine STR loci in the han population of Chengdu

作　　者：丁柳[1] 应斌武[1] 宋兴勃[1] 范红[1] 王军[1] 代波[1] 陆小军[1] 庄杰[1] 刘显中[1] 

机构地区：[1]四川大学华西医院实验医学科分子诊断实验室,四川成都610041

出　　处：《四川医学》2008年第10期1296-1298,共3页Sichuan Medical Journal

基　　金：国家自然科学基金(批准号30470761)资助

摘　　要：目的对成都汉族D3S1358、vWA、FGA、D8S1179、D21S11、D18S51、D5S818、D13S317及D7S820等9个STR基因座的遗传多态性进行群体遗传学研究。方法利用荧光标记复合扩增及毛细管电泳自动荧光检测的方法,对260名无关个体获得9个STR基因座等位基因的分布频率等群体遗传学数据。结果D3S1358、vWA、FGA、D8S1179、D21S11、D18S51、D5S818、D13S317及D7S820等9个基因座上分别检出等位基因6、9、12、9、17、15、9、8和9个。杂合度分别为:0.812、0.808、0.842、0.731、0.673、0.796、0.788、0.800和0.777;多态信息量为:0.830、0.800、0.850、0.750、0.680、0.750、0.770、0.840和0.760;个体识别率为:0.958、0.945、0.965、0.919、0.882、0.913、0.930、0.961和0.927;非父排除率为:0.621、0.613、0.680、0.4770、.388、0.592、0.578、0.599和0.577。9个STR基因座均符合Hardy-Weinberg平衡。结论这9个STR基因座多态性好,灵敏度高,可用于人类遗传分析及法医学中的亲子鉴定和个人识别。Objective To invest igate the allele frequencies of eight short tandem repeats（STR） loci： TH01, FES, D19S400, D7S820,D16S539,D20S161 ,D3S1545 and D5S818 in Han population in sichuan province. Methods Totally 260 unrelated individuals from majority population of Han Chinese in Chengdu have been inspected with 9 polymorphism STR loci D3S1358, vWA, FGA, D8S1179, D21S11,D18S51 ,D5S818,D13S317,D7S820 by means of capillary electrophoresis and fluorescence muhiple amplification. Results 6, 9,12,9,17,15,9,8 and 9 alleles of the 9 STR loci in Han population of Chengdu were detected. The measured values of the heterozygosity of the 9 STR loci were 0.812,0.808,0.842,0.731,0.673,0.796,0.788,0.800 and 0.777;of the polymorphism information content were 0.830,0.800,0.850,0.750,0.680,0.750,0.770.0.840 and 0.760;of the discrimination powcr（DP） were 0.958,0.945,0.965.. 0.919,0.882,0.913.0.930,0.961 and 0.927.The measured values of paternity exclusion were 0.621,0.613,0.680,0.477,0.388, 0.592,0.578,0.599 and 0.557;The genotype distributions were in accordance with Hardy-Weinberg equilbrium. Conclusion Our resuits showed that 6 STRs loci are valuable STRs loci genetic marker and useful in forensic medicine.

关 键 词：毛细管电泳 短串联重复序列(STR) 复合扩增 群体遗传学 

分 类 号：R394[医药卫生—医学遗传学] R392.11[医药卫生—基础医学]