Pep-1-sod对缺血再灌注沙鼠脑组织抗氧化能力的影响  

Antioxidative ability of Pep-1-sod on brain tissue of Mongolian gerbil undergoing cerebral ischemia-reperfusion

作　　者：周艳梅[1] 钟森[1] 董继平[1] 王小菊[1] 王金堂[1] 赵旸[1] 孙洁[1] 万斌[1] 

机构地区：[1]郧阳医学院附属十堰市人民医院儿科,湖北十堰442000

出　　处：《中国现代医学杂志》2008年第19期2779-2783,共5页China Journal of Modern Medicine

摘　　要：目的研究Pep-1-sod对缺血再灌注沙鼠脑组织的影响。方法将84只蒙古沙鼠随机分为假手术组(n=20)、缺血再灌组(n=20)、干预1组(n=23)及干预2组(n=21),测定不同时相沙鼠脑组织梗死体积、MDA水平、LDH水平及CAT活性。结果干预1组在不同时相的脑梗死体积均显著低于缺血再灌组及干预2组(分别为P<0.05和P<0.01),MDA及LDH水平均显著高于缺血再灌组及干预2组(分别为P<0.05和P<0.01),CAT活性均显著高于其他3组(分别为P<0.05和P<0.01)。结论Pep-1-sod可穿越血脑屏障对缺血再灌注沙鼠脑组织发挥抗氧化能力及脑保护作用。[Objective] To study the effects of Pep-1-sod on brain tissue of Mongolian gerbil undergoing cerebral ischemia-reperfusion. [Methodsl 84 Mongolian gerbils were used to made model and randomly divided into 4 groups, sham-operated group （n =20）, cerebral ischemia-reperfusion group （n =20）, Pep-1-sod treated group （n=23） and sod treated group （n=21）. Infarct volume, MDA level, LDH level and CAT activity of brain tissue of Mongolian gerbil were determined during 2, 6, 12, 24 and 48 h after ischemia-reperfusiun. [Results] The cerebral infarct volume of Pep-1-sod treated group was significantly lower than cerebral ischemia-reperfusion group and sod treated group （P〈0.05 and P 〈0.01, respectively）.In addition, cerebral MDA level and LDH level of Pep-1-sod treated group were significantly higher than cerebral ischemia-reperfusion group and sod treated group （P 〈0.05 and P〈 0.01, respectively）. Cerebral CAT activity of Pep-1-sod treated group was significantly higher than three of other groups （P〈0.05 and P 〈0.01, respectively）. [Conclusion] Pep-1-sod can cross through blood brain barrier and prevent brain tissue of Mongolian gerbils that undergo cerebral ischemia-reperfusion from oxidation, therefore can protect it from injuring.

关 键 词：细胞穿膜多肽 缺血再灌注 活性氧 

分 类 号：R-332[医药卫生]