维甲酸与人参皂甙-Rh_2或半边旗二萜类化合物5F协同诱导肝癌细胞凋亡的研究  

Studies on apoptosis of hepatocellular carcinoma cells induced by retinoic acid and ginsenoside-Rh2 or 5F from pteris semipinnata L

作　　者：蔡康荣[1] 蔡春[1] 

机构地区：[1]广东医学院分析中心,广东湛江524023

出　　处：《中国现代医学杂志》2008年第19期2788-2791,共4页China Journal of Modern Medicine

摘　　要：目的观察维甲酸与人参皂甙-Rh2或半边旗二萜类化合物5F协同诱导肝癌细胞凋亡及对Fas蛋白表达的影响。方法体外培养肝癌细胞,设RA、GS-Rh2、5F、RA+GS-Rh2、RA+5F3个实验组和对照组,分别收集其药物作用24和48h后的培养细胞,应用流式细胞仪(FCM)检测肝癌细胞的凋亡率及Fas蛋白的表达。结果肝癌细胞药物作用48h时,RA+GS-Rh2组、RA+5F组与RA组或GS-Rh2组或5F组比较凋亡细胞数和Fas蛋白的表达有显著增高(Р<0.05),但在24h时,RA+GS-Rh2组、RA+5F组与RA组比较"亚G1峰"差异无显著性(Р>0.05)。结论维甲酸与人参皂甙-Rh2或半边旗二萜类化合物5F协同作用可以增强诱导肝癌细胞凋亡,促进Fas蛋白的表达。[Objective] To observe the apoptosis of Hepatoeellular carcinoma cells induced by Retinoic acid（RA） and Ginsenoside-Rh2 （GS-Rh2） GS-Rh2.5F, or 5F from Pteris semipinnata L（5F） and to be the influence of the expression of Fas. [Methods] To establish RA, RA and GS-Rh2, RA and 5F groups and control groups. Hepatocellu-lar carcinoma ceils were treated with RA, RA and GS-Rh2, RA and 5F for 24 h and 48 h. The apoptosis and the expression of Fas in Hepatocellular carcinoma cells were detected by Flow cytometry （FCM）. [Results] Obvious apoptotic cells and expression of Fas were found when Hepatoeellular carcinoma cells were treated with the RA and GS-Rh2, RA and 5F for 48 h than in the RA（P 〈0.01）. No obvious apoptotie cell of sub-G1 were found in 24 h（P 〉 0.05）. [Conclusion] These results suggested that RA and GS-Rh2 or RA and 5F could enhance the apoptosis and expression of fas of Hepatocellular carcinoma cells.

关 键 词：肝癌细胞 维甲酸 人参皂甙-Rh2 半边旗 细胞凋亡 FAS蛋白 

分 类 号：R735.7[医药卫生—肿瘤]