猪HK2基因第17外显子多态与胴体和肉质性状的相关性研究  

Association of Single Nucleotide Polymorphisms in Exon 17 of Porcine HK2 Gene with Meat Quality and Carcass Traits

作　　者：王军[1,2] 邓昌彦[1] 熊远著[1] 左波[1] 李凤娥[1] 雷明刚[1] 郑嵘[1] 

机构地区：[1]华中农业大学农业部猪遗传育种重点实验室和农业动物遗传育种教育部重点实验室,武汉430070 [2]武汉科技学院环境科学研究所,武汉430073

出　　处：《畜牧兽医学报》2008年第10期1295-1299,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑计划(2006BAD01A08-01);国家“863”项目(2007AA10Z166);湖北省重大专项(2006AA202A01);湖北省自然科学基金(2005ABA142)资助

摘　　要：采用PCR-RFLP技术,分析了HK2基因第17外显子中MspⅠ的酶切突变多态位点(A171G)在6个群体186头猪中的多态性分布及其与胴体性状和肉质性状间的相关性。研究结果表明:3个纯种猪群和3个杂交猪群中A和B等位基因频率分布情况较一致,均为B等位基因占绝对优势;在6个群体中,没有发现AA基因型个体,AB与BB基因型效应与胴体长、肋骨数和眼肌面积的相关性极显著(P<0.01),与肩部背膘厚、胸腰椎间背膘厚、三点平均背膘厚、眼肌高度、眼肌宽度、肥肉率、瘦肉率和肌内脂肪等性状的相关显著(P<0.05)。该位点是否可用作改良猪胴体、肉质性状的标记位点并在标记辅助选择中加以应用还有待进一步的研究。Hexokinase （HK） Ⅱ is a key enzyme member in the glycolysis reaction which expressed mainly in muscle. In this study,the polymorphisms of Msp Ⅰ site （A171G） in the 17th exon of HK2 gene in 186 pigs of six pig populations were analyzsed by using PCR-RFLP, and the relationship between the HK2 genotypes and pork quality, carcass traits was evaluated. The results showed that the frequencies of the two alleles （A and B） were similar in all studied homozygous and heterozygous pig breeds, and allele B was dominant. In addition, no AA genetype was found in six breeds, the statistically associations of AB and BB with carcass length, rib numbers, and lion eye area were very significant different （P〈0.01）, but the significant differences （P〈 0. 05） of their genetype effects were found in backfat thickness at shoulder, backfat thickness at thorax-waist, and average backfat thickness over three positions, lion eye height, lion eye width, fat meat percentage, lean meat percentage and, intramuscular fat. It needs more and comprehensive studies to verify whether the polymorphic site could be used as the candidate site affecting carcass and meat quality traits in markers assisted selection.

关 键 词：猪 己糖激酶 PCR—RFLP 胴体性状 肉质性状 关联分析 

分 类 号：S828.2[农业科学—畜牧学]