日粮中添加天门冬氨酸镁对肉仔鸡肝脏和腿肌脂质过氧化状态的影响  

Effects of Dietary Magnesium Asparatate Supplementation on Lipid Peroxidation Status in the Liver and the Thigh Muscles of Broiler Chickens

作　　者：刘永祥[1,2] 刘艳丽[1] 黄炎坤[1] 呙于明[2] 

机构地区：[1]郑州牧业工程高等专科学校畜牧工程系,郑州450008 [2]中国农业大学动物科技学院,北京100193

出　　处：《畜牧兽医学报》2008年第10期1349-1354,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目(30371043)

摘　　要：研究日粮添加天门冬氨酸镁(MgAsp)对肉仔鸡肝脏和腿肌脂质过氧化状态的影响及其机理。将128只22日龄的AA肉仔鸡随机分配到对照组和加镁日粮组,每组8个重复,每个重复8只鸡,以玉米、豆粕为主要原料配制对照组日粮,在此基础上添加1%的MgAsp作为加镁组日粮。42日龄时,每个重复随机抽取1只鸡,颈静脉放血处死,收集血清、肝脏和腿肌等样品。试验结果表明,与对照组相比,加镁组肉仔鸡血清、腿肌和肝脏镁的含量分别提高了22%、21%和29%(P<0.01),加镁组肉仔鸡肝脏和腿肌中丙二醛(MDA)的含量分别降低了24%(P<0.01)和29%(P<0.01),加镁组肉仔鸡肝脏中过氧化氢酶(CAT)和总超氧化物歧化酶(SOD)的活性分别提高了25%(P<0.01)和14%(P<0.05),腿肌CAT的活性提高了37%(P<0.01),日粮添加MgAsp显著降低腿肌中活性氧(ROS)的含量(P<0.01)。两组肉仔鸡腿肌和肝脏中不饱和脂肪酸的总含量差异不显著(P>0.05)。上述结果说明,玉米-豆粕日粮中添加MgAsp可以有效降低肝脏和腿肌中MDA的产量;腿肌中CAT活性的提高和ROS产量的降低是日粮添加MgAsp降低腿肌中脂质过氧化水平的原因;肝脏中CAT和SOD活性的提高是日粮添加MgAsp降低肝脏中脂质过氧化水平的原因。The experiment was conducted to investigate the effects of dietary magnesium as paratate （MgAsp） supplementation on lipid peroxidation status in the liver and the thigh muscles of broiler chickens and its possible mechanisms. One hundred and twenty eight 22-day-old male AA broiler chickens were allocated into two treatments, each treatment with eight replicates of 8 chickens. The maize and soyabean meal are the main ingredients of diet in control group, and the diet supplemented with 1% MgAsp served as the diet of experimental group. At 42 days old, one of each replicate was slaughtered. Serum, thigh muscle and liver were collected. Results showed that, compared with the control group, the Mg concentration of serum, thigh muscle and liver in broilers fed with Mg supplementation diet increased by 22% 21% and 29% （P〈0.01）, respectively; Malondialdehyde （MDA） contents of liver and the thigh muscle decreased by 24% （P〈0.01） and 29%（P〈0.01）, respectively; Hepatic catalase （CAT） and total superoxide dismutase （SOD） activities increased by 25%（P〈0.01） and 14%（P〈0.05）, respectively; The thigh muscle CAT activity increased by 37% （P 〈0.01）; Dietary Mg supplementation significantly decreased reactive oxygen species （ROS） production in the thigh muscles（P〈0.01） ; There was no difference in the total content of unsaturated fat acids between two groups （P〉0.05）. These results suggested that MgAsp supplementation in the diets based on maize-soyabean could decrease lipid peroxidation level in the liver and the thigh muscles of broiler chickens; Enhanced CAT activity and decreased ROS production in the thigh muscle lead to the decrease of lipid peroxidation level, however it is the enhanced hepatic CAT and SOD activities not ROS production that decreased lipid peroxidation level in the liver of broiler chickens.

关 键 词：天门冬氨酸镁 肝脏 腿肌 脂质过氧化 肉仔鸡 

分 类 号：S831.5[农业科学—畜牧学]