氨氯地平对实验性兔动脉粥样硬化进程中一氧化氮合酶—一氧化氮和血红素氧合酶—一氧化碳系统的影响  

Effects of Amlodipine on Heme Oxygenase/Carbon Monoxide and Nitric Oxide Synthase/Nitric Oxide Systems in Atherosclerosis Model of Rabbits

作　　者：于清霞[1] 刘同涛[2] 孙春丽[2] 田庆印[2] 李博勤[3] 

机构地区：[1]烟台市毓璜顶医院ICU,山东省烟台市264000 [2]山东大学齐鲁医院心内科 [3]山东大学医学院电镜室,山东省济南市250012

出　　处：《中国动脉硬化杂志》2008年第8期615-618,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨血红素氧合酶—一氧化碳和一氧化氮合酶—一氧化氮系统在动脉粥样硬化中的变化、相互关系及氨氯地平对动脉粥样硬化进程中血红素氧合酶—一氧化碳和一氧化氮合酶—一氧化氮的影响。方法家兔予以高胆固醇饮食8周,8周后停用高胆固醇饮食或另外给予喂饲氨氯地平进行药物干预8周。结果与对照组比较,模型组血脂水平明显升高,血清一氧化氮含量明显降低,血浆一氧化碳水平明显升高,血红素氧合酶1及诱导型一氧化氮合酶表达明显升高(P均<0.01)。与模型组比较,氨氯地平组血浆胆固醇、甘油三酯、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇差异无显著性(P>0.05);血清一氧化氮含量差异亦无显著性(P>0.05),血浆一氧化碳水平明显降低(P<0.01),血红素氧合酶1及诱导型一氧化氮合酶表达明显减少(P<0.01)。结论动脉粥样硬化进程中,血红素氧合酶—一氧化碳和一氧化氮合酶—一氧化氮系统显示出互补及代偿性调节作用,氨氯地平可以通过下调血红素氧合酶—一氧化碳和一氧化氮合酶—一氧化氮系统而延缓动脉粥样硬化的进程。Aim To investigate the change nile and correlation of heme oxygenase-1 （HO-1）/ carbon monoxide（CO） and nitric oxide synthase（NOS） / nitric oxide（NO） and the influence of amloclipine on the two systems in atheroselerotie progress. Methods The rabbits received 1% cholesterol diet （ n = 16） for eight weeks; after the eight weeks,one group （ n = 8） were fed with normal diet for eight weeks and onther group （ n = 8） were administrated with amlodipine. Results Compared with contrast group, in model group the levels of serum hpids and plasma earbon monoxide were inereased obviously （ P 〈 0.01） ; however, the levels of serum nitrie oxide and the expression of heme oxygenase-1 mad indueible nitfie oxide synthase in aortie were deereased markedly （ P 〈 0.01 ）. Compared with model group , in amlodipine group the levels serum lipids and serum nitric oxide were no significant effects （ P 〉 0.05）, the levels of plasma carbon monoxide were decreased obvaously （ P 〈 0.01 ）, while the expression of heine exygenase- 1 and indueible nitric oxide synthase in aortic reduced greatly （ P 〈 0.01 ） . Conclusion In atheroselerotie progress, between heine oxygenase-I （HO-1）/carbon monoxide （CO） and nitfie oxide synthase （NOS）/nitfic oxide （NO） system appears the reciprocal relationship, and amledipine may delay atheroselerotie progress by dowhregulate the two systems.

关 键 词：病理学与病理生理学 动脉粥样硬化 血红素氧合酶 一氧化氮合酶 一氧化氮 一氧化碳 氨氯地平 免疫组织化学 

分 类 号：R363[医药卫生—病理学]