胶州湾多重耐药菌中整合子基因研究  

Study on integron in multidrug resistance isolates in Jiaozhou Bay

作　　者：王春艳[1] 党宏月[2] 丁永生[3] 

机构地区：[1]大连海事大学环境科学与工程学院 [2]中国石油大学(华东)生物工程与技术中心,山东青岛266555 [3]上海海事大学海洋环境与安全工程学院,上海200135

出　　处：《海洋环境科学》2008年第5期414-417,共4页Marine Environmental Science

基　　金：国家自然科学基金项目(40476058,40576069)

摘　　要：为调查整合子在胶州湾水域多重耐药菌中的分布情况。本文采用PCR、克隆测序等方法对多重耐药菌株中I、II型整合酶及整合子中耐药基因盒的分布进行检测。研究发现,胶州湾多抗性细菌污染严重50%的多重耐药菌携带整合子;整合子阳性菌株对多种抗生素的耐药率远大于整合子阴性菌;二氢叶酸还原酶和氨基糖苷腺苷转移酶是基因盒携带的主要耐药基因;基因盒-整合子系统参与了细菌多重耐药的形成,已成为水环境中多重耐药基因水平传播的重要机制。The investigate of distribution of integron in multidrug resistance isolates in Jiaozhou Bay was carried out. The presence of Class 1 and 2 integrase genes were determined by PCR with integrase specific primers. Clone and DNA sequencing were used to analyze the resistance-related gene cassettes. 50% of multidrug resistance isolates were detected with integron. The multidmg resistance rate of positive-integron isolates is far greater than the negative ones. The dfr and aadA were predominant genes in integron. The integron-mediated horizontal transference plays a particularly important role in the spread of mnhidrug resistance in aquatic environments.

关 键 词：水环境 多重耐药 整合子 

分 类 号：X172[环境科学与工程—环境科学]