添加电子载体耦联原位液液萃取的高效丁醇发酵  

High Performance Butanol Fermentation Coupled with In-situ Liquid-Liquid Extractive Technology by Adding Electron Carrier

作　　者：牛晓乐[1] 史仲平[1] 张龙云[1] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《安徽农业科学》2008年第26期11188-11189,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究添加电子载体耦联原位液液萃取技术构建更高效丁醇发酵系统的可能性。[方法]添加微量的中性红等电子载体,增加发酵过程的还原力、改变发酵代谢流和产物比例;添加电子载体与使用油醇作为原位萃取剂相耦联,进一步提高丁醇萃取发酵的性能。[结果]在使用浓度15%初始玉米醪的条件下,与单纯的萃取发酵相比,高效耦联丁醇萃取发酵的生产强度和油相中的丁醇浓度分别提高了24.5%和40.9%。[结论]中性红可以改变代谢流,促成"高丁醇/低丙酮"丁醇发酵的实现。[ Objective ] The research aimed to study the possibility of constructing more efficient fermentation system through the technology of in-situ liquid-liquid extractive fermentation by adding electron carrier. [ Method] Using oleyl alcohos as extractant and adding neutral red as electron carrier to improve the fermentation performance. [ Result] Under the condition of using 15% high corn powder medium, compared with extractive fermentation, the production intensity and the butanol concentration respectively increased by 24.5% and 40.9% when using that coupled with in-situ liquid-liquid extractive fermentation. [ Conclusion ] It could enhance reducing power , change the direction of metabolic flux and the ratio of production which promoted forming the fermentation mode of ＂high butanol / low acetone＂ adding neutral red.

关 键 词：萃取发酵 电子载体 丁醇 油醇 

分 类 号：TS262.5[轻工技术与工程—发酵工程] G202[轻工技术与工程—食品科学与工程]