多发性硬化及视神经脊髓炎患者外周血CD4^+CD25^+调节性T细胞的检测及其临床意义——附53例报告  

The detection and clinical significance of CD4^+CD25^+ regulatory T cell in peripheral blood of patients with multiple sclerosis and neuromyelitis optica

作　　者：熊英琼[1] 王朝东[1] 谢旭芳[1] 曹文峰[1] 张昆南[1] 吴晓牧[1] 

机构地区：[1]江西省人民医院神经内科,330006

出　　处：《新医学》2008年第10期639-641,共3页Journal of New Medicine

摘　　要：目的:探讨多发性硬化及视神经脊髓炎患者外周血CD4+CD25+调节性T细胞(regula-toryTcell,Treg)的水平及其临床意义。方法:采用流式细胞仪检测31例多发性硬化患者(多发性硬化组)、22例视神经脊髓炎患者(视神经脊髓炎组)及36名健康对照人(对照组)外周血中的CD4+CD25+CD127lowTreg,采用Flowjo流式细胞数据分析软件V7.2.4分析CD4+CD25+CD127lowTreg在CD4+T细胞中所占的比例,比较3组的差异。结果:多发性硬化组CD4+CD25+CD127lowTreg占CD4+T细胞的(4.0±1.6)%;视神经脊髓炎组相应为(3.0±0.5)%,对照组相应为(6.5±1.3)%,前2组患者的CD4+CD25+CD127lowTreg占CD4+T细胞的比例均明显低于对照组。结论:多发性硬化、视神经脊髓炎患者外周血CD4+CD25+Treg水平下降,这可能与该类疾病的发生有关。Objective： To study the level and clinical significance of CD4^＋ CD25^＋ regulatory T cell （Treg） in peripheral blood of patients with multiple sclerosis （MS） and neuromyelitis optica （NO）. Methods： CD4^＋ CD25^＋ CD127^lowTreg were detected with flow cytometry in peripheral blood of patients with MS （31 cases）, NO （22 cases） and healthy control group （36 cases）. The percentage of CD4^＋ CD25^＋ CD127^low Treg in CD4^＋ T cell was analyzed by Flowjo software V7.2.4. The difference was compared among the three groups. Results： In MS group, NO group and control group, the percentages of CD4^＋ CD25^＋ CD127^low WTreg in CD4^＋ T cell were （4. 0 ± 1.6）% , （3.0 ± 0. 5 ）% and （6. 5 ± 1.3 ）%. The percentages of CD4^＋ CD25^＋ CD127^low Treg in CD4^＋ T cell in MS group and NO group were both lower than that in control group. Conclusion： The level of CD4^＋ CD25^＋ Treg in peripheral blood decrease in MS and NO patients. This may contribute to the pathogenesis of the diseases.

关 键 词：CD4^+CD25^+调节性T细胞 自身免疫性疾病 脱髓鞘疾病 多发性硬化视神经脊髓炎 

分 类 号：R744.5[医药卫生—神经病学与精神病学]