β-环糊精荧光增敏的间苯二酚荧光分析法  

Detection of Resorcinol by Fluorescence Enhance of β-Cyclodextrin

作　　者：夏开豪[1] 孙向英[1] 刘斌[1] 李丹[1] 

机构地区：[1]华侨大学材料科学与工程学院,福建泉州362021

出　　处：《华侨大学学报（自然科学版）》2008年第4期550-553,共4页Journal of Huaqiao University(Natural Science)

基　　金：国家自然科学基金资助项目(20575023);福建省国际合作重点项目(2006I0021);福建省自然科学基金资助项目(D0710017;D0810016);国务院侨办科研基金资助项目(06QZR10)

摘　　要：利用β-环糊精对间苯二酚的包络作用,增敏间苯二酚的荧光强度,提出一种测定环境中间苯二酚的荧光检测新技术.考察各种因素对实验的影响,结果表明,β-环糊精对间苯二酚的荧光增敏作用在碱性环境下的效果比在酸性环境下的效果要好;NaOH、乙醇对β-环糊精增敏间苯二酚的荧光反应有很大的促进作用.实验最佳条件：β-环糊精浓度为0.01 mol·L^-1,NaOH质量浓度为0.3 g·L^-1,乙醇体积分数为1.4%.该方法测定间苯二酚简便、选择性好,且间苯二酚的浓度在0.091～0.909 mmol·L^-1范围内,和荧光强度有良好的线性关系,检测下限为27.3μmol·L^-1.Based on the β-cyclodextrins＇ fluorescence enhancement to resorcinol, a new way of fluorescence analysis for resorcinol is presented in this paper. The effects of various conditions are investigated, experimental results show that recognition of resorcinol by fluorescence enhanced with β-cyclodextrin is less pronouncing in the acidic system than in the alkaline system. In addition, NaOH and ethanol have great promotion to the fluorescence reaction. Optimal experimental conditions display the concentration of β-cyclodextrin is 0. 01 mol · L^-1 , that of NaOH is 0.3 g · L^-1, and the volume ratio of ethanol is 1.4%. The method has good selectivity and convenience in the detection of resorcinol. There is a linear relationship between the change of fluorescence with the concentration of resorcinot in the range of 0. 091 -0. 909 mmol· L^-1, and the detection limit was as low as 27. 3μmol· L^-1.

关 键 词：荧光分析 自组装膜 间苯二酚 Β-环糊精 包络作用 

分 类 号：O657.31[理学—分析化学]