NaCS/PDMDAAC微囊化肺炎克雷伯氏菌发酵产1,3-丙二醇  

Primary Study on Culture of Encapsulated Klebsiella pneumoniae for Production of 1,3-Propanediol in Shaking Flask

作　　者：陈国[1] 陈宏文[1] 彭益强[1] 方柏山[1] 

机构地区：[1]华侨大学工业生物技术福建省高等学校重点实验室,福建泉州362021

出　　处：《华侨大学学报（自然科学版）》2008年第4期554-558,共5页Journal of Huaqiao University(Natural Science)

基　　金：福建省自然科学基金资助项目(E0810017);福建省青年人才创新项目(2006F3080);华侨大学高层次人才科研启动项目(06BS214)

摘　　要：研究一种新型的生物微胶囊体系——NaCS/PDMDAAC,包埋肺炎克雷伯氏菌(K.pneumoniaeZJU5205)产1,3-丙二醇.比较不同初始菌体包埋量、胶囊/发酵液体积、发酵液pH值、初始甘油质量浓度等对微囊化细胞发酵结果的影响.结果表明,将细胞种子液稀释5倍后包埋,当胶囊与发酵液的体积比为1∶2、初始pH值为7、初始甘油质量浓度为60 g.L-1时,得到较好的发酵结果.考察肺炎克雷伯氏菌在囊内的生长曲线,以及底物和产物质量浓度随发酵时间的变化,发现由于胶囊引起的扩散限制,可以持续维持囊内较低的底物质量浓度,从而部分克服高质量浓度底物对菌体生长和生成产物的抑制.A novel biocapsule system composed of NaCS/PDMDAAC （sodium cellulose sulfate/poly[-dimethyl（diallyl） ammonium chloride]） was used to encapsulate the Klebsiella pneumoniae ZJU 5205 for the production of 1,3-propanediol. The effects of initial cell loading in capsules, volume ratio of capsules to medium, pH value of medium and initial glycerol concentration on the fermentation results were investigated. The better results were obtained at the following conditions： the concentration of seed culture was diluted to one fifth to encapsulate; volume ratio of capsules to medium was 1/2; initial pH was 7.0; initial concentration of glycerol was 60 g · L^-1. The cell growth, glycerol consumption, 1, 3-propanediol accumulation by encapsulated Klebsiella pneumoniae in capsules and in medium were described. The results showed that lower concentration of substrate in capsules which is caused by diffusion limitation of capsule membrane could partially overcome the inhibition of glycerol on cell growth and 1,3-propanediol producing.

关 键 词：生物微胶囊 细胞固定化 肺炎克雷伯氏菌 1 3-丙二醇 

分 类 号：Q814.2[生物学—生物工程] TQ923[轻工技术与工程—发酵工程]