两种构建大鼠膀胱癌模型方法的比较研究  

The Comparative Study in the Construction of Experimental Animal Model of Bladder Tumor in Rat induced by BBN and MNU.

作　　者：李云[1] 许传亮[1] 李志华 杨庆[1] 顾正勤[1] 王林辉[1] 孙颖浩[1] 

机构地区：[1]上海市第二军医大学附属长海医院泌尿外科,200433 [2]上海市市北医院泌尿外科,200435

出　　处：《中国医药指南（学术版）》2008年第19期24-26,共3页Guide of China Medicine

摘　　要：目的通过BBN喂饲和MNU膀胱灌注方法构建大鼠膀胱癌模型,比较两种实验方法的优缺点。方法BBN诱癌组大鼠使用0.05%BBN溶液连续喂养6周,后改用2%枸橼酸钠溶液作后续喂养22周,第28周为诱导的终点。MNU诱癌组大鼠行MNU膀胱灌注,每两周灌药一次,每次2mg/只,共4次,第10周为诱导的终点。比较两组大鼠的成瘤率和死亡率。结果BBN诱癌组30只大鼠喂养28周后存活16只,11只成瘤,病死率46.7%,存活大鼠成瘤率68.8%;MNU诱癌组30只大鼠第10周存活28只,25只成瘤,病死率6.7%,存活大鼠成瘤率89.3%。结论利用MNU行大鼠膀胱灌注的方法构建大鼠膀胱癌模型,具有成瘤时间短、成瘤率高、大鼠病死率低等特点,优于BBN喂饲大鼠成瘤的方法。Objective To compare the advantages and disadvantages in the construction of experimental animal model of bladder tumor in rat induced by BBN and MNU respectively. Methods A total of 30 female SD rats weighing about 180-220 gm were given drinking water contain 0.05%BBN for 6 weeks continuously, then fed 2% natrium citricum solution for 22 weeks, 28 weeks later all rats were killed. Other 30 female SD rats were infused intravesically with 2rag MNU 4 times, at 2 weeks interval, 10 weeks later all rats were killed, then the incidence of tumor growth and mortality of two groups were compared. Results BBN treatment： 28 weeks later 16 rats were alive and 11 of them had bladder tumor. MNU treatment： 10weeks later 28 rats were alive, bladder tumor were detected in 25 rats. Conclusion Intravescular administration of MNU in rats provided a useful model for experimental studies on bladder tumor, the incidence of tumor growth is higher and the mortality of rats is lower than BBN treatment.

关 键 词：化学致癌剂 N-正丁基-N-(4-羟基-丁基)亚硝胺 N-甲基亚硝基脲 动物模型 膀胱肿瘤 

分 类 号：R730.231.1[医药卫生—肿瘤]