胆结石形成早期几种胃肠激素变化的实验研究  

Experimental study on the changes of gastrointrstinal hormones during early stage of cholesterol gallstone formation

作　　者：王陵[1] 董蕾[2] 段炜[1] 杨姝洁[1] 

机构地区：[1]武警陕西总队医院呼吸消化科,陕西西安710054 [2]西安交通大学第二医院消化科,陕西西安710004

出　　处：《武警医学院学报》2008年第11期963-965,969,共4页Acta Academiae Medicinae CPAPF

摘　　要：【目的】探讨胆结石形成早期血浆MOT、5-HT、血清NO的变化。【方法】选取健康成年豚鼠45只,随机分3组:A组,喂普通饲料2周;B组,喂1%胆固醇饲料2周;C组,喂1%胆固醇饲料4周。分别应用放免法、荧光分光光度法、硝酸酶还原法检测血浆MOT、5-HT及血清NO浓度。【结果】B组喂食高胆固醇饲料2周后,豚鼠血浆MOT、5-HT水平升高(P<0.01);C组喂食高胆固醇饲料4周后,豚鼠血浆MOT、5-HT水平较A组升高(P<0.01),较B组也升高(P<0.05)。B组喂食高胆固醇饲料2周后,血清NO水平降低(P<0.01);C组喂食高胆固醇饲料4周后,血清NO水平较A组降低(P<0.01);较B组也降低(P<0.05)。【结论】胆结石形成早期,豚鼠血浆MOT水平增高,血浆5-HT水平升高,血清NO浓度降低。[ Objective] To detemaine the change of the concentration of motilin （MOT） in plasma, 5-hydroxytryptamine （5-HT） in plasma and nitric oxide （NO） in serum dynamically after recieving 1% （high） cholesterol diet. [ Methods] 45 healthy adult guinea pigs were included in the study and were randomly assigned to the following groups： Group A： fifteen animals received a trace （ control group） for two weeks. Group B： fifteen animals recieved a 1% （high） cholesterol diet for two weeks. Group C： fifteen animals daily received a 1% （high） cholesterol diet for four weeks. Plasma concentration of MOT, 5-HT and serum concentrafion of NO were measured. [Results] While recieving 1% （high） cholesterol diet for two weeks, the plasma level of MOT and 5-HT were significantly higher than that of the control group （ P 〈 0.01） and the serum NO level was lower significantly than that of the control group （ P 〈 0.01）. While recieving 1% （high） cholesterol diet for four weeks, the plasma level of 5-HT and serum MOT increased significantly as compared with the control group （ P 〈0.01） and with group B （ P 〈0.05）. While recieving 1% （high） cholesterol diet for four weeks, the serum NO level was lower significantly than that of the control group （ P 〈0.01） and that of group B （ P 〈0.05）. [Conclusions] During the early stage of cholesterol gallstone formation the plasma level of MOT is increased and the plasma level of 5-HT is higher as compared with the control group, white the NO level in serum is lower than that of the control group.

关 键 词：胆结石 胃动素 5-羟色胺 一氧化氮 

分 类 号：R575.6[医药卫生—消化系统]