多肿瘤标志物蛋白芯片检测对原发性肝癌的诊断价值  

The diagnositic value of testing tumor protein chip in primary liver cancer

作　　者：周秀[1] 张爱民[1] 靳颖[1] 王世钰[1] 张桂芬[1] 

机构地区：[1]武警医学院附属医院检验科,天津300162

出　　处：《武警医学院学报》2008年第11期991-993,共3页Acta Academiae Medicinae CPAPF

摘　　要：【目的】探讨12种肿瘤标志物(testing multi-tumor,TM)联合检测对原发性肝癌的诊断价值。【方法】应用蛋白芯片技术(C-12)定量测定62例原发性肝癌患者、53例良性肝病患者血清中糖类抗原-199(CA199)、神经原特异性烯醇化酶(NSE)、癌胚抗原(CEA)、糖类抗原-242(CA242)、铁蛋白(SF)、β-绒毛膜促性腺激素(β-HCG)、甲胎蛋白(AFP)、游离前列腺特异性抗原(Free-PSA)、前列腺特异性抗原(PSA)、糖类抗原125(CA125)、人生长激素(HGH)、糖类抗原-153(CA153)等12种TM的浓度。【结果】通过12种TM联合检测将原发性肝癌组阳性检出率提高到了100%;良性肝病组阳性检出率为22.6%;原发性肝癌组的TM测定值明显高于良性肝病组,有显著性差异,其中AFP最为显著(P<0.001),其次为CA199、CEA、CA125(P<0.01);CA242、SF、β-HCG(P<0.05)。两组比较无统计学意义为NSE、Free-PSA、PSA、HGH、CA15-3(P>0.05)。原发性肝癌组TM阳性检出率由高到低依次为:AFP>CA199>SF>CA125>CEA>CA242>β-HCG>CA153。【结论】应用12种TM联合检测原发性肝癌、良性肝病,可以为临床诊断提供更多的指标,提高辅助诊断的准确性,弥补单一TM特异性不强和敏感性较低的不足,有效地筛选出肿瘤高危人群,对原发性肝癌的早期发现、早期诊断具有重要意义。[ Objective] To study the diagnositic value of testing multi - tumor protein chip （C-12） in primary liver cancer. [Methods] Detected the concentrations of serum tumor makers of glucide - antigen199 （CA199）, neuronspecific enolase （NSE）, earcinoemb-ryonic antigen （CEA）, glucide-antigen242 （CA242）, serum-ferritin （SF）, β-human chorionic gonadotrophin （β- HCG）, alpha- fetoprotein （AFP）, free prostatic gland specific antigen （Free- PSA）, prostatic gland specific antigen （PSA）, glucide - antigen125 （CA125）, human growth hormone （HGH）, glucide - antigen153 （CA153） in 62 primary liver cancer patients, 53 hepatocirrhosis patients and 70 healthy controls. [Results] The positive detection rate is 100% in primary liver cancer group, 22.6% in hepatocirrhosis group, it has the statistical significances between the two groups, AFP （ P 〈0.001）, CA199, CEA, CA125 （ P 〈0.01）, CA242, SF, β- HCG （ P 〈0.05）, the positive detection rate in primary liver cancer group from high to low is AFP 〉 CA199 〉 SF 〉 CA125 〉 CEA 〉 CA242 〉β - HCG 〉 CA153. [ Conclusions] C- 12 can be effectively used for screening tumor high- risk group, they are valuable in early finding, early diagnosis and early treatment for primary liver cancers..

关 键 词：肿瘤标志物 原发性肝癌 肿瘤蛋白芯片 

分 类 号：R735.7[医药卫生—肿瘤]