罗布麻叶提取物对四氧嘧啶诱发的糖尿病小鼠肾功能降低的影响  

Effect of Extract from Apocynum Venetum L on Depression of Renal Function in Diabetic Mice

作　　者：杨新波[1] 黄正明[2] 陈红艳[1] 王建华[1] 耿淼[1] 杨坤[1] 曹文斌[1] 

机构地区：[1]解放军总医院老年医学研究所,北京100853 [2]解放军第302医院,北京100039

出　　处：《解放军药学学报》2008年第5期408-411,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：全军十一五中医药推广项目;No.2006213002

摘　　要：目的观察罗布麻叶提取物对四氧嘧啶诱发的早期糖尿病小鼠血糖、血脂及肾功能的影响。方法给小鼠尾静脉注射四氧嘧啶65mg·kg-1造成糖尿病模型,按血糖值分成5组:分别是罗布麻叶提取物小、中、大(50、100、200 mg·kg-1)3个剂量组,阳性药二甲双胍组(150mg·kg-1)和模型对照组,另设1组正常对照(共6组)。连续灌胃治疗4周。末次给药后3h眼球取血。用自动生化分析仪测定血糖、甘油三酯、胆固醇、肌酐、尿素氮及胰淀粉酶。为探讨作用机制建立体外非酶糖基化反应系统,采用竞争性AGE-ELISA方法测定非酶糖基化终末产物(AGE)。结果罗布麻叶提取物可明显对抗糖尿病升高的尿素氮和肌酐,大剂量时可降低血糖和甘油三酯。体外实验显示有强大的非酶糖基化抑制作用。结论罗布麻叶提取物对糖尿病早期肾功能降低有明显保护作用,该作用可能与其抑制非酶糖基化反应减少AGE形成有关。Aim To observe the extract from Apocynum Venetum L （AVE） on blood glucose , blood lipids and renal function of early diabetic mice. Methods The mice were injected through the caudal vein with alloxan 65 mg·kg^-1 to induce a diabetic model. The diabetic mice were divided into five groups treated with AVE ：50,100,200mg·kg^-1, mefformin 150mg·kg^-1 and model control,while a normal control group was set up simultaneously. The drugs were given ig for four weeks. Three hours after the last treatment, the blood was taken by exposing the eye ball. Blood glucose （Glu）, triglyceride （Trig）, cholesterol （Chol）, creatinine （ Cre ）, blood uria nitrogen （BUN） and pancreatic amylase （Amy） were determined by Auto-biochemistry Analysis Meter. The reaction system of nonenyzymatic glycation （NEG） was set up in order to explore the mechanism of AVE , and advanced glycation end products （AGE） were assayed by AGE- ELISA method, Results After treatment with AVE, Glu and lipids decreased slightly in the large dose group, while Cre and BUN was reduced notablely. The experiment also showed that AVE inhibited AGE in vitro significantly. Conclution AVE could antagonise the renal functioninjuries in the early diabetic model. This action may be associated with the inhibition of AVE on reaction of nonenyzymatic glycation （NEG） and formation of AGE.

关 键 词：罗布麻叶提取物 糖尿病 肾功能 非酶糖基化 

分 类 号：R965[医药卫生—药理学]