动态浊度法定量测定克林霉素磷酸酯氯化钠注射液中细菌内毒素的含量  

Quantitative Detection of Bacterial Endotoxin in Clindmycin Phosphate Sodium Chloride Injection by Kinetic Turbidimetric Assay

作　　者：王方[1] 宋晓红[1] 

机构地区：[1]解放军第153中心医院药剂科,河南郑州450042

出　　处：《解放军药学学报》2008年第5期451-453,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的探讨建立动态浊度法定量测定克林霉素磷酸酯氯化钠注射液中细菌内毒素的方法。方法参照中国药典2005年版附录中细菌内毒素检查法及细菌内毒素检查法应用指导原则的规定,对克林霉素磷酸酯氯化钠注射液中细菌内毒素定量测定进行可行性研究。结果克林霉素磷酸酯氯化钠注射液经8倍稀释后可完全消除其对鲎试剂与细菌内毒素凝集反应的干扰。结论应用动态浊度法定量测定克林霉素磷酸酯氯化钠注射液中细菌内毒素含量,结果准确,在实际应用中完全可行。Aim To establish a quantitative method for detecting bacterial endotoxin in Clindmycin phosphate sodium chloride injection through kinetic turbidimetric assay. Methods A study was performed of the feasibility of quantitative detection of bacterial endotoxin in Clindmycin phosphate sodium chloride injection,based on the appendix of China Pharmacopoeia 2005. Results Diluting the Clindmycin phosphate sodium chloride injection for 8 times can completely elimi- nate its interference with the TAL preparations. Conclusion Quantitative detection of bacterial endotoxin in Clindmycin phosphate sodium chloride injection by kinetic turbidimetric assay is entirely feasible in practice.

关 键 词：克林霉素磷酸酯氯化钠注射液 动态浊度法 细菌内毒素 

分 类 号：R927.2[医药卫生—药学]