溶菌酶在羊毛织物上的固定化  

Immobilization of lysozyme on wool fabric

作　　者：胡英俊[1] 范雪荣[1] 王强[1] 王平[1] 崔莉[1] 

机构地区：[1]江南大学生态纺织教育部重点实验室,江苏无锡214122

出　　处：《毛纺科技》2008年第10期15-18,共4页Wool Textile Journal

基　　金：国家"863"项目(项目编号:2007AA02Z218)

摘　　要：以羊毛织物为载体,研究了戊二醛交联固定溶菌酶的工艺条件。结果表明,戊二醛交联工艺的最佳条件为:戊二醛0.2%,交联时间2 h。溶菌酶固定化的条件为:溶菌酶5 g/L,固定化时间6 h,固定化pH值7.0,固定化温度4℃。在此工艺条件下制得的固定化溶菌酶的操作稳定性较好,使用6次后相对酶活仍保持在30%左右。The optimum conditions for immobilization of lyzozyme with wool fabric and glutaraldehyde were studied. The optimum conditions of the crosslinking with the glutaraldehyde were： wool fabric was cross-linked with 0.2% glutaraldehyde for 2 h. The optimum immobilization process was as follows： lysozyme 5 g/L, immobilization time 6 h, immobilization pH value 7.0, immobilization temperature 4 ℃. On this case, the operational stability of immobilized lysozyme was well, there still remained about 30% enzyme activity after six times using.

关 键 词：溶菌酶 羊毛织物 固定化 戊二醛 交联 

分 类 号：TS195.56[轻工技术与工程—纺织化学与染整工程]