宫颈癌组织中MHC-Ⅰ类抗原呈递相关蛋白CNX HSP90 GRP94的表达及意义  

The Expression and Possible Diagnostic Significance of MHC ClassⅠAntigen Presentation Associated Proteins(CNX,HSP90,and GRP94)in Human Cervical Cancer

作　　者：郝治[1] 拉莱.苏祖克 海米提.阿布都力木 杜靖[1] 

机构地区：[1]新疆医科大学厚博学院机能教研室,乌鲁木齐市830011 [2]新疆医科大学基础医学院病理解剖教研室 [3]新疆医科大学基础医学院组织胚胎学教研室

出　　处：《中国肿瘤临床》2008年第19期1109-1111,1116,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金资助(编号:30460046)

摘　　要：目的:探讨宫颈炎和宫颈癌组织中CNX、HSP90、GRP94蛋白和HPV16感染在维吾尔族和汉族妇女中的表达及相关关系。方法:采用免疫组化SP法和PCR技术检测宫颈炎组织66例(汉族39例,维吾尔族27例)以及宫颈癌组织92例(汉族30例,维吾尔族62例)中CNX、HSP90、GRP94蛋白的表达和HPV16的感染情况。结果:CNX蛋白在维、汉宫颈癌组织中的阳性率低于维、汉宫颈炎组织中阳性率表达(67.39%,86.36%)(P<0.05)。并且,CNX蛋白的阳性率在宫颈炎组织中维吾尔族高于汉族(100%,76.92%)(P<0.05)。HSP90和GRP94蛋白在维、汉宫颈癌组织中的阳性率表达高于维、汉宫颈炎组织(77.17%,56.06%;77.17%,60.60%)(P<0.05)。宫颈癌组织中HSP90蛋白阳性率表达维吾尔族低于汉族(74.19%,83.33%)(P<0.05)。HPV16感染在宫颈炎及宫颈癌中的阳性率均为维吾尔族高于汉族(P<0.05)。通过相关性研究发现,在维吾尔族妇女宫颈癌组织中HSP90与GRP94呈正相关性(r=0.249,P<0.05)。HSP90蛋白的阳性表达率与HPV16型感染在宫颈癌组织中呈显著正相关(r=0.361,P<0.05)。结论:通过对CNX、HSP90、GRP94蛋白和HPV16联合检测能提高宫颈癌的检出率。Objective： To evaluate of the expression of the MHC class I antigen presentation-associated proteins CNX, HSP90 and GRP94, and to investigate their correlation with HPV16 infection in cervicitis and cervical cancer in patients of Urghur and Hart nationality. Methods： We used immunohistochemistry to detect the expression of CNX, HSP90 and GRP 94 proteins in 66 cervicitis tissues from 39 Han and 27 Urghur women and 92 cervical cancer tissues from 30 Han and 62 Urghur women. Using specific primers for HPV16, PCR was employed to detect HPV16 infection in these tissues. Results： The overall expression rate of CNX in cervical cancer tissues （67.39 %） in both ethnic groups was lower than that in cervicitis tissues （86.36 %） （P〈0.05）. The expression rate of CNX in the cervicitis tissues from Urghur women was higher than that in Han women （100% vs. 76.92%, P〈0.05）. The expression of HSP90 and GRP94 in the cervical cancer tissues from both Urghur and Han women was higher than that in the cervicitis tissues （P〈0.05）. The expression rate of HSP90 in the cervical cancer tissues was lower in Urghur patients than in Han patients （P〈0.05）. The infection rate of HPV16 in cervicitis and cervical cancer in Urghur women was higher than that in Han women （P〈0.05）. A correlation was found between HSP90 expression and GRP94 expression in cervical cancer in Urghur patients （r=0.249, P〈0.05） .The expression of HSP90 was positively correlated with HPV16 infection in cervical cancer in both ethnic groups （r=0.361, P〈0.05）. Conclusion： Detection of CNX, HSP90, and GRP94 protein expression along with screening for HPV16 infection may improve the diagnosis of cervical cancer.

关 键 词：宫颈癌 乳头状瘤病毒 CNX HSP90 GRP94 

分 类 号：R737.33[医药卫生—肿瘤] R711.6[医药卫生—临床医学]