氧诱导对新生大鼠视网膜NF—κB表达的影响  

The expression of nuclear factor-kappaB in oxygen-induced retinopathy of newborn rats

作　　者：周岚[1] 田兆方[2] 杨军[2] 

机构地区：[1]淮安市第二人民医院眼科,江苏淮安223002 [2]南方医科大学珠江医院儿科中心,广东广州510282

出　　处：《中国实用眼科杂志》2008年第10期1147-1149,共3页Chinese Journal of Practical Ophthalmology

摘　　要：目的探讨核因子κB（nuclear factor-kappaB，NF—κB）与新生大鼠视网膜病变的关系。方法以75％的氧浓度暴露1周转入空气1周的方法建立新生大鼠视网膜病变模型共16只，同时设正常对照大鼠16只。在视网膜病变模型结束后及1周时，组织学观察视网膜毛细血管密度指数（Retinal Capillary Density Index，RCDI）的改变，免疫组织化学技术评估视网膜NF-κB的活性变化。结果两组视网膜RCDI比较，相同时点两组间差异有显著性（t=11．08，P=0．0000；t=3．06，P=0．0008），而同组不同时点比较显示：高氧组P21较P14显著减少（t=5．94，P=0．0000）；而对照组P21与P14间差异无显著性（t=0．49，P=0．6288）。NF-κB的活性比较，相同时点两组间差异有统计学意义（t=16．75，P=0．0000；t=14．93，P=0．0000），而同组不同时点比较显示，高氧组P21较P14显著减少（t=8．29，P=0．0000）；而对照组P21与P14间差异无统计学意义（t=0．34，P=0．7409）。结论氧诱导新生血管的发生，NF—κB的活性在氧诱导新生大鼠视网膜病（oxygen-induced retinopathy）中显著增强，随病程的延长而逐渐减少，视网膜NF—κB的表达与新生血管的发生相关。Objective To investigate neovascularization （NV）and the associated changes in the expression of NF-κB in OIR（oxygen-induced retinopathy）.Methods 32 newborn Sprague-Dawley rats were raised in 2 expanded litters. Beginning at day 1 of life, rats were exposed to 7 daily cycles of hyperoxia （75% O2）. Rats were sacrificed after a subsequent 7-day room air recovery period. An additional 16 rats raised in room air served as age matched controls. The eyeballs of 8 rats were enucleated and fixed by formaldehyde at postnatal 14 days （P 14 ）and postnatal 21 days （P21 ）respectively, and then cross sectioned and stained with hematoxylin and eosin （HE）. The nuclei of proliferative retinal vessels were counted through the crosssections to measure the average retinal capillary density index （RCDl）.Right eyes in OIR litters and room air controls were processed for analysis of NF-κB by immunohistochemistry.Results There exist obviously differencc between two groups in the field of both RCDI and the expression ofNF-K B at the same time point（ t =11.08, P =0.0000; t =3.06, P =0.0008 ） （ t = 16.75, P =0.0000; t = 14.93, P =0.0000 ）.When RCDI and the expression of NF-K B were copared between the different time point within the same groups （ t =11.08,P =0.0000; t = 3.06, p=0.0008 ）, there exist obviously differencc between two time point in the experiment group（t=5.94, p=0.0000 ; t =8.29,P =0.0000）,but no differencc in the control group （t =0.34,P =0.7409;t =0.34,P =0.7409）. Conclusions Neovascularization resulting from OIR occurs before room air recovery.NF-κB was upregulated and subsequently downregulated in OIR.Neovascularization in the OIR model appear to be associated with increased retinal NF-κB.

关 键 词：视网膜病变 核凶子κB 氧诱导 

分 类 号：R363[医药卫生—病理学] R587.2[医药卫生—基础医学]