重复经颅磁刺激对帕金森病鼠纹状体生长相关蛋白和突触素表达的影响  

The effect of repetitive transcranial magnetic stimulation on the expressions of growth associated protein-43 and synaptophysin in striatum of Parkinson's disease model mice

作　　者：王全懂[1] 顾平[1] 董巧云[1] 王彦永[1] 刘力[1] 王铭维[1] 

机构地区：[1]河北医科大学第一医院神经内科河北省脑老化与认知神经科学重点实验室,河北石家庄050017

出　　处：《中国老年学杂志》2008年第19期1885-1888,共4页Chinese Journal of Gerontology

基　　金：河北省自然科学基金资助项目(C007000842)

摘　　要：目的探讨重复经颅磁刺激(repetitive transcranialm agnetic,rTMS)对帕金森病(PD)模型鼠纹状体生长相关蛋白(Growth activty protein-43,GAP-43)和突触素(synaptophysin,p38)表达的影响。方法40只雄性C57BL/6J小鼠随机分为:对照组,PD模型组,假磁刺激(srTMS)组,rT-MS组,每组10只。小鼠皮下注射MPTP(15mg.kg-1.2h-1.次-1,注射4次)复制PD模型,rTMS干预,利用免疫组织化学染色技术检测GAP-43和p38的表达变化,并借助图像分析系统对其进行定量分析。结果PD模型组、srTMS组及rTMS组GAP-43阳性产物较对照组明显增多,以rTMS组GAP-43表达升高最为明显,且着色深,PD模型组和srTMS组校正光密度值(COD)均明显高于对照组(P<0.05),rTMS组COD显著高于对照组(P<0.01)及PD模型组和srTMS组(P<0.05)。PD模型组和srTMS组及rTMS组p38表达较对照组反应产物明显减少,PD模型组和srTMS组COD均显著低于对照组(P<0.01),rTMS组COD值明显低于对照组(P<0.05),但rTMS组p38表达较PD模型组和srTMS组增多,COD显著高于PD模型组和srTMS组(P<0.05)。结论rTMS可诱导受损的纹状体区GAP-43和p38的表达上调,从而推测rTMS可能通过促进轴突再生和突触重塑,进而对受损的多巴胺转运通路起修补作用,增加多巴胺转运,从而达到治疗作用。Objective To investigate the effect of repetitive transcranial magnetic stimulation （rTMS） on the expressions of growth activity protein （GAP）-43 and synaptophysin （p38） in striatum of Parkinson＇s disease （PD） model mice. Methods 40 male C57BL/6J mice were randomly divided into control,PD model, sham rTMS, rTMS groups （n = 10）. The PD model of C57BL/6J mice were made by subcutaneous injection of MPTP solution （ 15 mg · kg-^-1·2 h^-1 ） and then were treated with rTMS for 14 days. The GAP-43 and p38 expressions in striatum were detected by immunohistochemical method, and the contents of GAP-43 and p38 were analyzed by image analysis system. Results The positive reactions of GAP-43 apparently increased in striatum of PD, srTMS and rTMS groups, and the corrected optical denstities （COD） were higher significantly than those of control group （ PD and srTMS groups （ P 〈 0. 05 ）, rTMS group（ P 〈 0. 01 ）. Positive reactions of GAP-43 of rTMS group apparently increased comparing with that of PD and srTMS groups, the COD of rTMS group were higher significantly than that of PD and srTMS groups （P 〈 0. 05 ）. Positive reactions of p38 apparently decreased in striatum of PD, srTMS and rTMS groups, COD of immunologic reactions of p38 of PD, srTMS and rTMS groups were decreased significantly than that of control group[（ PD and srTMS groups, P 〈 0. 01 ）, （ rTMS group P 〈 0. 05 ） ], but positive reactions of p38 of rTMS group apparently increased than that of PD and srTMS groups, the COD of rTMS group were higher significantly than that of PD and srTMS groups （P 〈 0. 05）. Conclusions rTMS could upregulate expression of GAP-43 and p38 in striatum of PD model mice, may play a role in treating PD by inducing synapse remodeling and subsequent to repair dopamine transport pathway.

关 键 词：重复经颅磁刺激 纹状体 生长相关蛋白 突触素 

分 类 号：R742.5[医药卫生—神经病学与精神病学]