FHIT基因在原发性皮肤T细胞淋巴瘤中的异常改变及意义  

Detection and Significance of Abnormality FHIT Gene Exons in Cutis Primary T Cell Lymphoma

作　　者：田中伟[1] 宋向凤[2] 马慧群[3] 牛新武[3] 冯捷[3] 彭振辉[3] 

机构地区：[1]新乡医学院第一附属医院皮肤科,河南卫辉453100 [2]新乡医学院基础医学院免疫教研室,河南新乡453003 [3]西安交通大学第二医院皮肤科,陕西西安710004

出　　处：《中国皮肤性病学杂志》2008年第10期584-586,共3页The Chinese Journal of Dermatovenereology

基　　金：新乡医学院科研启动基金资助(编号:200612001)

摘　　要：目的检测原发性皮肤T细胞淋巴瘤中脆性组氨酸三联体(FHIT)基因的转录情况。方法用PCR-SS-CP法检测了10例原发性皮肤T细胞淋巴瘤患者皮损FHIT基因外显子5和8的缺失和突变状况,10例健康皮肤组织做对照。结果10例肿瘤组织中,FHIT基因外显子5缺失者7例,FHIT基因外显子8的缺失者2例;而正常人FHIT基因外显子5和8无1例缺失;对扩出的外显子进行SSCP分析,未检测到其突变。结论原发性皮肤T细胞淋巴瘤中存在FHIT基因外显子5和8的异常缺失,这种异常转录可能与该病的发生有关。Objective To detect deletion and mutation of exon 5 and 8 of FHIT gene in cutis primary T cell lymphoma, and to analyze the role of the abnormality in the carcinogenesis of cutis cancer. Methods The deletion and mutations of exon5,8 of FHIT gene were detected in 10 cutis primary T cell lymphoma samples tissues by polymerase chain reaction and single-strand conformation polymorphism （ PCR - SSCP） methods. Results Deletion of exon 5 was observed in 7 out of 10 lymphoma samples, and the deletion of exon 8 was 2 out of 10 lymphoma. No mutation was found in exon 5,8 of FHIT gene. Conclusion Deletion of exon 5,8 was observed in cutis primary T cell lymphoma and no mutation was found. The abnormality may play a role in the pathogenesis of the cutis primary T cell lymphoma.

关 键 词：原发性皮肤T细胞淋巴瘤 FHIT PCR—SSCP 

分 类 号：R739.5[医药卫生—肿瘤]