P21活化激酶1蛋白在大肠癌及大肠腺瘤异型增生中的表达及意义  

P21-activated kinase 1 protein expression and its significance in colorectal carcinoma and colorectal dysplastic adenoma

作　　者：谭卫民[1] 罗时敏[1] 葛轶群[1] 庄思敏[1] 罗建威[1] 

机构地区：[1]广州市第一人民医院综合外科,510180

出　　处：《中国医师进修杂志（外科版）》2008年第10期10-12,共3页Chinese Journal of Postgraduates of Medicine

基　　金：基金项目：广东省科技计划项目（63084）;广州市医药卫生科技项目（2006-YB-203）

摘　　要：目的探讨p21活化激酶1（PAK1）蛋白与大肠癌发生及生物学行为的关系。方法采用免疫组化染色法对10例正常大肠黏膜、40例大肠绒毛状或管状腺瘤及60例大肠癌组织进行标记分析。结果PAK1蛋白在正常大肠黏膜中的阳性率为10．0％，在大肠腺瘤伴轻、中、重度异型增生中的阳性率分别为25．0％、33．3％和33．3％，在大肠癌中的阳性率为65．0％。大肠癌PAK1蛋白阳性率高于正常大肠黏膜（P〈0．01）及大肠绒毛状或管状腺瘤（P〈0．01），且高于伴轻度异型增生腺瘤（P〈0．01）及伴中度异型增生腺瘤（P〈0．05）。低分化大肠癌患者PAK1蛋白阳性率高于高分化大肠癌患者（P〈0．05）；而有淋巴结转移者PAK1蛋白阳性率高于无淋巴结转移者（P〈0．05）；Dukes分期中的C期和D期患者PAK1蛋白阳性率高于A期患者（P〈0．05）。结论PAK1蛋白的表达与大肠癌的发生有一定的相关性，可作为判断大肠癌生物学行为及预后的一个有用指标。Objective To investigate the role of p21-activated kinase 1 （PAK 1 ） in colorectal mucosal careinogenesis and the relationship between PAK 1 expression and biological behavior of colorectal carcinoma. Method PAK1 was detected with method in 10 normal colorectal mucosas, 40 colorectal villous or tubular adenomas and 60 colorectal carcinomas. Results The positive rate for PAK 1 was 10.0% in normal colorectal mucosas, and 25.0%, 33.3% and 33.3% in slight, moderate and severe dysplastic adenomas, respectively, 65.0% was found in colorectal carcinomas. The positive rate for PAK1 in colorectal carcinomas was higher than that in normal colorectal mucosas（P 〈 0.01 ）, colorectal villous or tubular adenomas （P 〈 0.01 ）, slight dysplastic adenomas （P 〈 0.01 ） and moderate dysplastic adenomas （P 〈 0.05 ）. The positive rate for PAKlof poor differentiated colorectal carcinomas was higher than that of high differentiated ones （P 〈 0.05 ）, and the positive rate for PAK 1 of patients with lymph node metastasis was higher than that of patients without lymph node metastasis （P 〈 0.05 ）. In clinical stages, the positive rate for PAK1 in Dukes C and D stages patients was higher than that in Dukes A stage patients, respectively （P 〈0.05）. Conclusion PAK1 maybe play some role in the process of carcinogenesis of colorectal mucosa, and be used as an useful marker for assessment of the biological behavior and prognosis of colorectal carcinoma.

关 键 词：结直肠肿瘤 p21活化激酶1 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤] R735.34[医药卫生—临床医学]