都匀亚洲带绦虫不同方式感染宿主的免疫病理变化  

Immunopathological observation of pig infected with Taenia saginata asiatica by different ways

作　　者：杨丽[1] 李溥[1] 沈振华[2] 欧燕芳[2] 党荣敏[1] 

机构地区：[1]黔南民族医学高等专科学校基础医学部,贵州都匀558003 [2]黔南民族医学高等专科学校第二附属医院病理科

出　　处：《中国病原生物学杂志》2008年第10期754-757,共4页Journal of Pathogen Biology

基　　金：贵州省黔南州自然科学基金重点资助项目(No.2007008);黔南民族医学高等专科学校科研专项资金资助(No.QNYZ2007005)

摘　　要：目的建立等量都匀亚洲带绦虫虫卵单次感染和多次感染的动物模型,比较两种不同感染方式对家猪肝脏的损害程度。方法将实验动物猪分成3组,单次感染组、多次感染组和正常对照组,分别灌喂都匀亚洲牛带绦虫的孕节,感染动物于3、9、18周剖杀,制作病理切片,测量单个囊尾蚴肉芽肿面积,放射免疫法检测家畜血清中透明质酸(hyal-uronic acid,HA)和层粘连蛋白(laminin,LN),免疫组化法检测肝内结缔组织生长因子(connective tissue growth factor,CTGF)与转化生长因子-β1(transforming growth factor-betal,TGF-β1)蛋白的表达水平。结果单次感染组家猪急性期囊尾蚴肉芽肿反应最明显,慢性期和晚期肉芽肿面积逐渐缩小,而多次感染组肉芽肿缩小不明显;血清中HA和LN、肝组织中CTGF和TGF-β1水平在感染各期均高于正常对照组,多次感染组升高更显著。结论用等量的都匀亚洲带绦虫虫卵多次感染家猪比单次感染引起的肝纤维化更严重。Objective To compared the liver damages in swine induced by single and multiple infection with same number of Taenia sagenata asiatica. Methods The experimental animals were divided into 3 groups： single infection groups, multiple infection groups and the control group, and fed with gestational segment of T. sagenata asiatica from Duyun, and killed at the 3th, 9th, 18th weeks, respectively. The size of cysticerei granuloma was measured in the pathological sections of liver, hyaluronic acid （HA） and laminin （LN） in sera were detected by RIA, and the hepatic expression of CTGF and TGF-β1 was detected by immunohistochemical method. Results The granuloma response in liver appeared to be rather prominent in acute stage with large size of cysticerci granuloma, but they become smaller during chronie or latter stage in the single infection group, while the shrinking of granuloma was so prominent in multiple infection groups. The contents of HA and LN in sera and CTGF and TGF-β1 in liver tissues were all higher than those of the control group, but the elevation was even more prominent in multiple infection group. Conclusion It is concluded that the multiple infection with same numbers of cysticercous cercaria can elicit greater damages to the host than that of the single infection.

关 键 词：亚洲牛带绦虫 肝纤维化 人工感染 猪 免疫病理变化 都匀 

分 类 号：R383.3[医药卫生—医学寄生虫学]