流动注射化学发光法测定哈西奈德  

FI-Chemiluminescence Determination of Halcinonede

作　　者：白浩[1] 吕九如[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,西安710062

出　　处：《理化检验（化学分册）》2008年第10期927-929,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：试验发现，肾上皮质激素药物哈西奈德对鲁米诺与N-溴代丁二酰亚胺（BSMD）在碳酸钠-碳酸氢钠缓冲介质中反应所产生的化学发光（CL）有明显增强作用，且其增强程度与此药物的质量浓度在1．0×10^-6～1．0×10^-4g·L^-1之间呈线性关系，检出限（3S／N）为3×10^-7g·L^-1。根据上述结果并结合应用流动注射技术，提出了测定哈西奈德的流动注射化学发光方法。在浓度水平为4．0×10^-5g·L^-1条件下，按方法作11次平行试验，求得其相对标准偏差为3．0％。应用此法分析了3个哈西奈德药物的实样，并用药典方法进行对照试验。结果表明：两方法所得结果相符。The chemiluminescence （CL） produced by the reaction between luminol and N-bromosuccinimide （BSMD） in Na2CO3-NaHCO3 buffer medium was found to be strongly enhanced by the presence of halcinonede, an adrenocorticomimetic drug, and the magnitude of increase in CL-intensity was found to keep linear relationship with the concentration of halcinonede in the range of 1.0 × 10^-6 - 1.0 ×10^-4 g · L^-1, with a detection limit （3S/N） of 3 × 10^-7g · L^-1. Based on these facts and by applying the flow injection technique, a method of FI-CL determination of halcinonede in its preparation was proposed. Precision of the method was tested at the concentration level of 4.0×10^-5g · L^-1 by 11 determinations, and the value of RSD obtained was 3. 0%. In analyzing 3 substantial samples of the drug, results obtained by this method were in consistency with the results found by the method of pharmacopoeia

关 键 词：化学发光测定 流动注射技术 N-溴代丁二酰亚胺 鲁米诺 哈西奈德 

分 类 号：O657.31[理学—分析化学]