云南江城和黑井盐矿沉积物未培养放线菌多样性比较  

Comparison of Actinobacterial Diversity in Jiangcheng and Heijing Saline Mines in Yunnan by Using Culture-independent Approach

作　　者：吴晋元[1] 职晓阳[1] 李岩[1] 关统伟[2] 唐蜀昆[1] 徐丽华[1] 李文均[1] 

机构地区：[1]云南大学云南省微生物研究所教育部微生物多样性可持续利用重点实验室,昆明650091 [2]塔里木大学新疆兵团塔里木盆地生物资源保护利用重点实验室,阿拉尔843300

出　　处：《微生物学通报》2008年第10期1550-1555,共6页Microbiology China

基　　金：国家“973项目”(No.2004CB719601);国家自然科学基金项目(No.30600001);教育部科学技术重点项目(No.206139);教育部新世纪优秀人才支持计划(No.NCET-05-0821);云南省应用基础研究计划面上项目(No.2007C167M)

摘　　要：类群特异性引物的应用使得研究者可以对感兴趣的微生物类群进行针对性研究。围绕云南江城和黑井两个地区的3个盐矿样点沉积物中放线菌的多样性和群落组成,我们通过放线菌特异性引物对总DNA进行16SrRNA基因扩增,经过克隆文库构建,利用酶切并选择其中不同带型的133个克隆的16SrRNA基因插入片段进行测序。系统发育分析和统计学结果表明,两地放线菌16SrRNA基因克隆广泛分布于整个放线菌门,同时发现部分序列可能属于放线菌的新类群。分析结果还预示,江城和黑井两地盐矿虽处云南不同地域含盐区,但两地未培养放线菌物种多样性和系统发育关系均较为相似。Actinobacterial diversity in samples of saline mines was investigated by constructing three actinobacterium-specific 16S rRNA gene clone libraries from Jiangcheng salt mine and Heijing brine pits. The primers for the Class Actinobacteria were used to construct the 16S rRNA gene clone libraries. At least 469 purified clones were digested with Hae Ⅲ for RFLP analysis, in order to separate the clones into groups according to their restriction patterns. Among of them, 133 sequences were selected and determined for their partial 16S rRNA gene sequences. The result of phvlogenetic and statistic analwes indicated that actinobacterial OTUs were closely related to most members of the Class Actinobacteria, and some OTUs may represent some unknown groups. Heijing and Jiangcheng located in different saline places of Yunnan, China.However, the communities, species diversity of Actinobacteria and Phylogenetic relationship from the two saline mines were similar.

关 键 词：盐矿 极端环境 放线菌多样性 免培养技术 

分 类 号：Q93[生物学—微生物学]