高效液相色谱荧光检测法测定人血清中胆汁酸含量  

HPLC Determination of Bile Acids in Sera with Fluorescence Detector

作　　者：汪海林[1] 冯埃生[1] 邹汉法[1] 李瑞江[1] 张津[1] 赵瑞环[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心

出　　处：《分析测试学报》1997年第5期18-21,共4页Journal of Instrumental Analysis

摘　　要：应用反相高效液相色谱－荧光检测技术，发展了人血清中１４种胆汁酸分析的新方法，流动相中加入β环糊精作为添加剂，等度冲洗，一次分析可在６０ｍｉｎ内完成，且可以达到基线分离。由于胆汁酸的紫外吸收弱，采用４－溴甲基－７－甲氧基香豆素作为荧光试剂衍生。激发波长３２０ｎｍ，发射波长３８０ｎｍ。血清样品经预处理，回收率介于６６０％～９１６％之间。在００２５～１５ｍｇ／Ｌ质量浓度范围，色谱峰面积对浓度作线性回归分析，回归系数介于０９９０８～０９９８６之间。方法快速、简单、灵敏度高。A new HPLC method for the determination of free and conjugated bile acids in human sera has been developed Because of their weak UV absorption,bile acids are derivatized with the fluorescence reagent 4 bromomethyl 7 methoxycoumarin The excited wavelength is 320 nm,while emission wavelength is 380 nm β Cyclodextrin is used as an additive in the mobile phase Upon isocratic elution,baseline separation can be achieved and analyses can be completed within one hour Recoveries of human bile acids in serum sample varied from 66 0% to 91 6% and the RSD were 9 3%～21 5% for free and glycine conjugated bile acids The linear regression coefficients of 0 9908～0 9986 can be obtained,when peak areas are plotted versus injected amounts of free and glycine conjugated bile acids The method is simple,rapid,and sensitive

关 键 词：胆汁酸 Β-环糊精 荧光检测 高效液相色谱 

分 类 号：Q592.9[生物学—生物化学]