检测胃癌患者外周血中K-ras和p53基因突变的临床价值  

Significance of p53 and K-ras Gene Mutation of Peripheral Blood in Diagnosis of Gastric Carcinoma

作　　者：陈积贤[1] 张洁[2] 黄建武[3] 李红智[2] 豆长明[2] 余铭[1] 林道浙[1] 陈永康[1] 张仁虎[1] 吴伟力[1] 陈谦[1] 林峰[3] 

机构地区：[1]温州医学院附属第三医院肿瘤外科,浙江省温州市325200 [2]温州医学院生物中心 [3]温州医学院附属第三医院检验中心

出　　处：《中国全科医学》2008年第21期1944-1945,1953,共3页Chinese General Practice

摘　　要：目的探讨检测胃癌患者外周血中K-ras和p53基因突变的临床意义。方法应用聚合酶链反应-单链构象多态性分析(PCR-SSCP)法,对我院收治的73例胃癌患者外周血中的K-ras和p53基因突变进行检测,并与20例非肿瘤疾病患者作对照。结果73例胃癌患者中有7例外周血中检测出p53基因突变,阳性率为9.6%;5例检测出K-ras基因突变,阳性率为6.8%。对照组外周血中均未检出K-ras和p53基因突变。有无肝转移的胃癌患者外周血p53和K-ras基因突变阳性率间差异均有统计学意义(P<0.05),不同分化程度及临床分期的胃癌患者外周血p53和K-ras基因突变阳性率间差异无统计学意义(P>0.05)。结论PCR-SSCP法检测外周血中K-ras和p53基因DNA有助于胃癌肝转移的诊断。Objective To investigate clinical significance of p53 and K - ras mutation of peripheral blood in patients with gastric carcinoma. Methods Totally 73 patients with gastric carcinoma were chosen to detect gene mutation of p53 and K - ras with PCR - SSCP technology, and another 20 cases of non - tumor patients were enrolled as the controls. Results Among 73 patients, there were 7 who have p53 mutation in peripheral blood, with positivity rate ofg. 6%, and 5 who have K - ras mutation, with a pesitivity rate of 6. 8%. No p53 and K -ras mutation was found in peripheral blood of the controls. There was a significant difference in positivity rate of the p53 and K - ras mutation between the patients who have liver metastasis and who have not （ P 〈0. 05）, however, there was no significant difference in positivity rate of the p53 and K -ras mutation between the patients with different cancer grades of differentiation and clinicopathologic stages （ P 〉 0.05 ）. Conclusion Detection of gene mutation of cancer cell in peripheral blood by PCR - SSCP has a certain value to early diagnosis of hepatic micrometastasis in gastric carcinoma.

关 键 词：胃肿瘤 肿瘤转移 K—ras P53 

分 类 号：R735.2[医药卫生—肿瘤]