Ca^(2+)对水稻Ac/Ds愈伤组织增殖·分化及生根的影响  

Effect of Ca^(2+) on Proliferation,Differentiation and Rooting of Callus of Rice with Ac/Ds Elements

作　　者：袁云香[1] 万志刚[2] 石丹[2] 白建江[2] 孙丙耀[2] 

机构地区：[1]渭南师范学院,陕西渭南714000 [2]苏州大学生命科学学院,江苏苏州215006

出　　处：《安徽农业科学》2008年第27期11658-11659,11763,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30571049)

摘　　要：[目的]研究Ca2+对水稻Ac/Ds愈伤组织增殖、分化及生根的影响。[方法]选用水稻品种Dongjin的Ac/Ds插入突变株系的种子,从成熟胚盾片诱导出愈伤组织,在筛选出的最适增殖、分化及生根培养基中添加不同浓度的Ca2+,研究Ca2+对水稻愈伤组织增殖、分化及生根的影响。[结果]适当增加Ca2+浓度能提高水稻愈伤组织的相对生长量、分化率、再生植株生根条数及根长,当Ca2+浓度为6.0mmol/L时效果最佳,当Ca2+浓度为7.5~15.0 mmol/L时水稻愈伤组织的相对生长量、分化率、再生植株的生根条数及根长呈下降趋势。[结论]适当增加培养基中Ca2+浓度可提高水稻Ac/Ds愈伤组织的相对生长量、分化率、再生植株生根条数及根长。[Objective] The study was to research the effect of Ca^2＋ on proliferation,differentiation and rooting of callus of rice mutants with Ac/Ds.[Method] The seeds of rice mutants with Ac/Ds elements inserted from rice variety Dongjin were selected and the callus were induced from scutellum of mature embryo.Ca^2＋ with different concn.was added into the selected optimum enrichment,differentiation and rooting medium to study the effect of Ca^2＋ on proliferation,differentiation and rooting of rice callus.[Result] The relative growth rate and differentiation rate of rice callus and the rooting number and root length of regenerated plant could be increased when Ca^2＋ concn.was increased properly,and the effect was better when Ca^2＋ was 6.0 mmol/L,but the relative growth rate and differentiation rate of rice callus and the rooting number and root length of regenerated plant showed downward trend when Ca^2＋ was 7.5-15.0 mmol/L.[Conclusion] The relative growth rate and differentiation rate of callus of rice mutants with Ac/Ds elements and the rooting number and root length of regenerated plant could be increased when Ca^2＋ concn.was increased properly.

关 键 词：水稻 CA^2+ 增殖 再分化 生根 

分 类 号：S511[农业科学—作物学]