香蕉MaPRMT1基因的分离及表达分析  

Isolation and Expression Analysis of MaPRMT1 Gene in Banana

作　　者：刘凡[1] 张建斌[1] 贾彩红[1] 杨景豪[1] 徐碧玉[1] 金志强[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所

出　　处：《安徽农业科学》2008年第27期11671-11673,11676,共4页Journal of Anhui Agricultural Sciences

基　　金：公益性行业(农业)科研专项(nyhyzx07-029)

摘　　要：[目的]为研究香蕉果实采后成熟机理奠定分子基础。[方法]通过抑制差减杂交和cDNA微阵列相结合的方法分离获得1个香蕉PRMT基因的cDNA片断,并克隆了该基因全长cDNA序列,对其进行生物信息学分析和在香蕉不同器官与果实不同成熟时期的表达模式分析。[结果]MaPRMT1基因cDNA全长1158 bp,具有完整的开放阅读框,编码385个氨基酸,与植物中的PRMT的同源性较高,含有1个Methyltransf-11结构域。在香蕉根、茎、叶和果实中均有表达,且在茎和叶中的表达量较高。随采后天数的增加,表达量逐渐下降,但至香蕉乙烯释放高峰时最大,后又开始下降。[结论]MaPRMT1属于第1类精氨酸甲基转移酶,在香蕉不同器官和果实不同成熟期呈差异表达。[Objective]The aim of experiment was to lay molecular foundation for studying maturity mechanism of banana after harvest.[Method]The combined method of suppressing subtractive hybridization and cDNA micro-array was used to obtain cDNA segment of one PRMT gene in banana and the whole cDNA sequence of the gene was cloned.The bioinformatics analysis was operated on it,in addition,the expression profile analysis was conducted in different organs and different mature periods of banana.[Result]The whole length of cDNA in MaPRMT1 was 1 158bp and possessed a complete open reading frame,which could encode 385 amino acids.It had high with PRMT in plant,containing one Methyltransf-11 domain.The MaPRMT1 gene was expressed in root,stem,leaf and fruit of banana and the expression levels in stem and leaf were relatively high.As the increase of days after harvest,the expression level declined gradually,however it reached maximum when ethylene release was biggest,then it declined.[Conclusion] MaPRMT1 belonged to the first kind of arginine methyltransferase and it was expressed differently in different organs and in fruits in diferent mature periods.

关 键 词：香蕉 蛋白质精氨酸甲基转移酶(PRMT) 香蕉蛋白质精氨酸甲基转移酶1(MaPRMT1) 基因差异表达 RT-PCR 

分 类 号：S668.1[农业科学—果树学]