肝刺激因子刺激大鼠肝细胞表皮生长因子受体基因表达的初步研究  

Effects of hepatic stimulator substance on EGF receptor gene expression in rat hepatocytes

作　　者：安威[1,2] 

机构地区：[1]首都医科大学实验中心 [2]德国爱尔兰根-纽伦堡大学医学院肝脏研究室

出　　处：《中国病理生理杂志》1997年第5期504-508,共5页Chinese Journal of Pathophysiology

摘　　要：以超速离心技术制备大鼠肝刺激因子。采用无血清培养技术培养大鼠肝细胞并观察肝刺激因子作用肝细胞后不同时刻细胞表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）受体ｍＲＮＡ表达的变化，以探讨该因子作用的分子机制。结果显示：肝刺激因子可诱导ＥＧＦ受体ｍＲＮＡ的表达升高，在给药后２～６小时达高峰。肝刺激因子与ＥＧＦ合用可明显刺激受体ｍＲＮＡ的表达。Ｎｏｒｔｈｅｒｎ杂交分析结果表明，受体ｍＲＮＡ表达增加主要是三种转录本形式中的两种，即５．６和１０．０ｋｂ所造成。提示该因子可在转录水平调节ＥＧＦ受体活性。A liver-specific growth factor-hepatic stimulator substance(HSS)was prepared from weanling rats and the effect of HSS on regulation of EGF receptor mRNA expression was investigated in serum-free conditional medium of rat hepatocytes.The results showed that HSS stimulated expression of EGF receptor mRNA.The overexpression of the mRNA remained 2～24 hours after addition of HSS and EGF.At 2 and 6 hours,combination of HSS and EGF showed synergistic effect on regulation of EGF receptor mRNA.Northern blot analysis indicated the increases in the expression of 5.6 and 10.0kb transcripts mRNA,implying that HSS might influence the activity of EGF at the molecular and genetic level.This study demonstrated that proliferative action of HSS on hepatocytes might through its modulation on EGF receptor expression and receptor activity.

关 键 词：表皮生长因子 肝刺激因子 肝损伤 大鼠 

分 类 号：R575.02[医药卫生—消化系统]