绞股蓝总皂甙抗大鼠海马结构缺血再灌注损伤的实验研究  

Inhibitory effect of gynostemma pentaphyllum againt ischemia reperfusion damage of hippocampal struction in rat

作　　者：王秋桂[1] 胡松林[1] 何丽娅[1] 江翠娥[1] 雷亚宁[1] 林永生[1] 

机构地区：[1]咸宁医学院神经组化研究室

出　　处：《中国病理生理杂志》1997年第5期513-516,共4页Chinese Journal of Pathophysiology

摘　　要：探讨大鼠海马结构缺血再灌注损伤机理及绞股蓝皂甙抗缺血再灌注损伤的作用。结果：ＩＲ组海马组织中ＭＤＡ含量高于ＳＯＣ组（Ｐ＜０．０１），而ＳＯＤ含量低于ＳＯＣ组（Ｐ＜０．０１），差异非常显著；ＩＲ＋ＧＰ组海马组织中ＭＤＡ含量明显低于ＩＲ组（Ｐ＜０．０１），而ＳＯＣ含量明显高于ＩＲ组（Ｐ＜０．０１），ＩＲ组海马组织Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ，Ｃａ２＋－ＡＴＰａｓｅ的活性明显低于ＳＯＣ组（Ｐ＜０．０１），ＩＲ＋ＧＰ组Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ、Ｃａ２＋－ＡＴＰａｓｅ活性基本恢复。同时电镀观察神经组织的损害也有明显差异。提示绞股蓝总皂甙能提高ＳＯＣ的活力，减少脂质过氧化物的生成，减轻海马结构缺血再灌注损伤。其作用机理与抗氧化作用，改善ＡＴＰ酶的功能有关。In order to investigate the mechanism of ischemia reperfusion (IR) injury of hippocampal structure and the protective effect of gynostemma pentaphyllum(GP) on the neuronal damage,tissue superoxide dismutase (SOD)activity,ATPase activity,malondialehyde(MDA) content and ultrastructure changes in the hippocampus were observed.The result showed that in comparing with ischemia reperfusion group,hippocampal SOD activity was higher(P<0.01).MDA content was lower(P<0.01)and ATPase activity was higher in the IR+GP and sham operated control group.In addition,the neurous tissue damage observed under electron microscope also showed notable discrepancy.The result suggested that GP could increase SOD activity,reduce lipid peroxidation,reduce neuronal damage in the IR injury of hippocampal structure.

关 键 词：海马 绞股蓝总皂甙 脑缺血 再灌注损伤 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学] R364.12[医药卫生—临床医学]