转小鼠金属硫蛋白(MT)基因烟草不同器官中金属离子铅和锌的分布  

Distribution of Pb and Zn in Transgenic Metallothionein Tobacco

作　　者：生吉萍[1,2] 李汉臣 刘开朗[1] 茹炳根[2] 申琳[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]北京大学生命科学学院,北京100871 [3]河北职业师范技术学院食品工程系,河北秦皇岛066600

出　　处：《光谱学与光谱分析》2008年第10期2401-2403,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30671471,30571296)资助

摘　　要：转金属硫蛋白(MT)基因植物具有清除重金属污染的能力,但是金属离子在植物体内的分布研究较少。文章以多代培育纯合的转MT烟草为材料,研究了转MT基因烟草积累铅和锌以及铅和锌在不同器官的分布情况。结果表明转基因植株的老叶、茎部和根部铅和锌积累量显著高于普通烟草植株,整株的铅和锌含量分别提高了21.8%和27.2%。铅和锌在植物体内的分布也发生了变化,转基因植株老叶和根中的铅含量分别高出普通烟草同位置的30.2%和47.8%,锌在转基因植株老叶、茎和根中的含量分别高出普通烟草同位置的44.7%,29.2%和21.6%,说明铅更容易在转基因植株老叶和根中积累,而锌更容易在转基因植株的老叶和茎中积累。Transgenic metallothionein （MT） plant can clear the heavy metals from soil and environment, but the distribution of metals in plants has not been studied systematically. The Pb and Zn contents in different parts of transgenic MT tobacco plant of sixth generation and traditional plant （same culture variety as control） were analyzed. The Pb and Zn contents in total transgenic plant were 21.8% and 27. 2% higher than control, respectively. The distribution of Pb and Zn in different organs varied in these two types of plants. The Pb and Zn contents in old leaves, stem and root in transgenic plants were significantly higher than those in wild type tobacco, while there was no significant difference in young leaves. The Pb contents in old leaves and root were 30. 2% and 47.8% higher than those in the control, and the Zn contents in old leaves, stem and root were 4. 7%, 29. 2% and 21.6% higher than those in the control. These data showed that Pb was accumulated in old leaves and root easily, while Zn was accumulated in old leaves and stem easily.

关 键 词：转基因烟草 金属硫蛋白 铅 锌 分布 

分 类 号：O644.1[理学—物理化学]