甲啡肽,纳络酮对红藻氨酸诱发大鼠癫痫活动的影响  

Effects of metenkephalin and naloxone on kainic acidinduced epilepsy in rats

作　　者：高恩泉 廖维宏[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所第三研究室

出　　处：《第三军医大学学报》1997年第6期513-515,共3页Journal of Third Military Medical University

摘　　要：目的：探索甲啡肽（ＭＥ）、纳络酮（ＮＬＸ）对红藻氨酸（ＫＡ）诱发大鼠癫痫发作的行为和组织学效应。方法：大鼠经戊巴比妥钠腹腔麻醉后固定于ＳＮ－１型立体定位仪上，按大鼠海马定位图谱把直径为０．５ｍｍ的金属导管植于海马双侧ＣＡ１区：用微量注射器将ＭＥ、ＮＬＸ于１０ｍｉｎ内注入，随即腹腔内注射ＫＡ１０ｍｇ／ｋｇ或１２ｍｇ／ｋｇ。海马组织石蜡切片、ＨＥ染色，显微镜观测。结果：海马内微量注入ＭＥ可易化癫痫的发生，加重海马组织损伤。ＮＬＸ可拮抗ＭＥ的作用，减轻海马组织损伤。结论：内源性阿片肽在ＫＡ诱发大鼠癫痫发作中，对海马的兴奋性的调节起重要作用。Objective: To investigate the behavioral and histological effects of metenkephalin (ME) and naloxone (NLX) on kainic acidinduced epilepsy in rats. Methods: After the rats were anesthetized with intraperitoneal injection of sodium pentobarbital (30 mg/kg), their heads were mounted onto a SN1 stereotaxic apparatus (Narishige). Guiding cannula with a tip diameter of 0.5 mm was slowly implanted in CA1 area of the hippocampus (APO:3.0; RL:3.0; HO:3.0) on both sides. ME and NAL were microinjected through the implanted cannula into the bilateral CA1 areas of awake rats within 10 min. Intraperitoneal injection of 10 mg/kg or 12 mg/kg was immediately conducted. The extracted hippocampal tissue was embedded with paraffin and sectioned. The sections were then stained with HE and observed with microscopy. Results: Intrahippocampal microinjection of ME augmented epileptogenic action and neurotoxic effects of kainic acid (KA) while NLX retarded them. Conclusion: The endogenous opioid peptides may play an important role in regulating hippocampal excitability in KAinduced epilepsy in rats.

关 键 词：甲啡肽 纳络酮 红藻氨酸 癫痫 大鼠 海马 

分 类 号：R742.1[医药卫生—神经病学与精神病学]