缢蛏线粒体DNA提取方法的比较  

Comparison of isolation methods for Sinonovacula constricta mitochondrial DNA

作　　者：郑润玲[1] 李家乐[1] 牛东红[1] 

机构地区：[1]上海水产大学生命科学与技术学院,上海200090

出　　处：《分子科学学报》2008年第5期312-315,共4页Journal of Molecular Science

基　　金：国家"863"计划项目(2006AA10A410);上海市重点学科建设资助项目(Y1101);福建海洋与渔业局重点资助项目(闽海渔2007015)

摘　　要：分离缢蛏肌肉组织，液氮磨碎后，用3种方法提取线粒体DNA，紫外分光光度法定量．用琼脂糖凝胶电泳和线粒体COI基因PCR扩增产物鉴定所提取的线粒体DNA．结果显示OD260／OD280均在1．69-1．85之间．高盐沉淀法提取的线粒体DNA量最多．To compare the isolation methods of sinonovacula constricta mitochondrial DNA, three methods were used including the alkaline lysis procedure, proteinase K method and high-concentration-salt precipitation method to find the most suitable one. The isolated mtDNA was identified by UV spectrophotometer.Agarose gel electrophoresis and polymerase chain reaction of mitochondria gene COI were used to identify the obtained mtDNA. The purity of mtDNA was 1.69 - 1.85 from OD260/OD280, high-concentration-salt precipitation method obtains the highest quantity of mtDNA. High-concentration-salt precipitation method is suitable for the isolation of Sinonovacula constricta mtDNA, for it is easy to operate and have a considerable amount.

关 键 词：线粒体 缢蛏 MTDNA 

分 类 号：Q785[生物学—分子生物学]