大鼠急性心肌缺血后肌浆网RyR_2 mRNA表达变化  

Changes of RyR_2 mRNA Expression in Rats Suffering from Acute Myocardial Ischemia

作　　者：揭娅[1] 朱少华[1] 蒋艳伟[2] 张磊[1] 范氾[1] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]天津市刑事科学技术研究所,天津300193

出　　处：《法医学杂志》2008年第5期327-329,338,共4页Journal of Forensic Medicine

基　　金：湖北省自然科学基金资助项目(2007ABA293)

摘　　要：目的研究大鼠急性心肌缺血后心肌肌浆网兰尼碱受体蛋白2(ryanodine receptor 2,RyR2)mRNA表达的变化。方法将SD大鼠分为正常对照组、心肌缺血组和缺血性猝死组,采用腹腔注射垂体后叶素的方法复制大鼠急性心肌缺血和猝死模型,对心肌进行半定量荧光RT-PCR检测,观察RyR2 mRNA表达水平的变化。结果与正常对照组相比,不同时间和不同程度的急性心肌缺血后心肌肌浆网RyR2 mRNA表达均显著降低(P<0.05)。结论心肌缺血性损伤可诱导心肌钙调控蛋白RyR2 mRNA表达下调。Objective To investigate the changes of ryanodine receptor 2（RyR2） mRNA expression in rats suffering from acute myocardial ischemia. Methods SD rats were divided randomly into normal control group, myocardial isehemia group and sudden death group. The models of myocardial ischemia and sudden cardiac death were induced by intraperitoneal injection of hypophysine. The changes of RyR2 mRNA expression in cardiac sarcoplasmic reticulum （SR） of rats suffering from myocardial isehemia were detected by fluorescent RT-PCR technique. Results The levels of RyR2 mRNA in the myocardial ischemia group and sudden death group were significant lower than those in the control group（P〈0.05）. Conclusion Myocardial ischemia may induce down-regulation of cardiac SR RyR2 mRNA expression.

关 键 词：法医病理学 心肌缺血 兰尼碱受体钙释放通道 逆转录聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤] R542.2[医药卫生—临床医学]