重组毕赤酵母SMD1168/pGAPZaA-VAP1稳定性及发酵表达WSSV-VAP1  

Stability of recombinant SMD1168/pGAPZaA-VAP1 and its expression of WSSV-VAP1 by fermentation

作　　者：刘庆慧[1] 陈婷[1] 黄倢[1] 

机构地区：[1]农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《海洋水产研究》2008年第5期95-99,共5页Marine Fisheries Research

基　　金：国家高技术发展计划项目(2006AA100312);重点基础研究项目(2006CB101801)资助

摘　　要：研究发酵时间和接种量对WSSV-VAP1重组菌蛋白表达量的影响及重组毕赤酵母中外源基因的稳定性。结果表明,构建的重组菌SMD1168/pGAPZaA-VAP1在发酵72h后,蛋白表达量占总蛋白10.45%,控制接种量对基因工程菌Pichia pastoris表达WSSV-VAP1非常重要。外源基因的插入对宿主菌的生长未产生明显的影响,重组质粒在毕赤酵母SMD1168中很稳定。The effect of inoculation and fermention time on WSSV-VAP1 expression in recombinant Pichia pastoris was studied. The result showed that the percentage of expression protein was 10. 45% of total supernant protein. The volume of inoculation was an important factor for WSSV-VAP1 expression. Stability of WSSV-VAP1 gene in recombinant P. pastoris showed that the transformation of WSSV-VAP1 gene had little effect on the growth of the host cells. The recombinant plasmid had excellent stability in P. pastoris SMDl168.

关 键 词：WSSV—VAP1 重组毕赤酵母 稳定性 发酵 

分 类 号：Q176[生物学—水生生物学]