脑卒中康复期线栓SD大鼠模型的改良制备和死亡原因浅析  

Establishment of Focal Cerebral Ischemia Model with Modified Suture-occluded Method in Period of Rehabilitation and Analysis for Death Cause

作　　者：赵春禹[1] 钮竹[1] 张通[1] 潘立东[1] 裴志胜[1] 

机构地区：[1]中国康复研究中心北京博爱医院,北京市100068

出　　处：《中国康复理论与实践》2008年第10期916-918,F0003,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家"十五"重点攻关项目[No.2001BA703B18(B)

摘　　要：目的建立一种简便可靠、创伤较小的脑卒中康复期线栓动物模型，并分析模型死亡的相关原因。方法雌性SD大鼠209只分为传统手术组63只、改良手术组109只和手术对照组37只。传统手术组采用Zea-Longa法，从左颈外动脉（ECA）经颈内动脉分叉进入左颈内动脉（ICA）；改良手术组只结扎左颈总动脉（CCA），并从CCA下线栓；手术对照组只结扎左CCA不下线栓。所有实验动物观察时间2个月。结果两个月存活比例分别为：改良手术组54．13％、传统手术组31．75％，两组动物的术后神经功能评分差异无显著性意义（P〉0．05），但前者的手术时间明显缩短（P〈0．01），术后存活时间明显延长（P〈0．01）；大脑中动脉阻断大鼠死亡率高，多在术后12～48h，约占总死亡率的81％。结论本实验采用的改良手术方法制备线栓SD大鼠模型具有稳定可靠、存活期长等优点，符合脑卒中康复期基础研究的要求；实验大鼠死亡的主要原因是术后大面积脑水肿，其次是围手术期水电解质失衡。Objective To compare the reliability of modificatory method and traditional method in preparing ischemic stroke rat model in rehabilitation period. Methods 173 female SD rats were divided into traditional method group （n= 63）, modificatory method group （n=109） and control group （n=37）. In the traditional method group, the thread embolus was inserted through the left external carotid artery （ECA）, and then left ECA and pterygopalatine artery （PPA） were ligated. In the modificatory method group, the thread embolus was inserted through the left internal carotid artery （ICA） and only left common carotid artery （CCA） was ligated. In the control group, the thread embolus was not inserted, only CCA was ligated. Results There was no significant difference in neu-rofunction score between the modificatory method group and the traditional method group （P〉0.05）. The operating time was significantly shortened （P〈0.01） and the survival time was significantly prolonged in the modificatory method group compared with that of the traditional method group （P〈0.01）. The two-month survival rate was 54. 13% in the modificatory method group and 31.75% in the traditional method group receptively, the former was significantly longer than the latter （P〈0.01）. Conclusion The ischemie stroke model established by modificatory methods is not only precisely and reliable but also save operating time and improve survival rate of the animals compared with the traditional method. The main death causes of animals are large-area cerebral edema and electrolyte imbalance after operation.

关 键 词：脑卒中康复期 SD大鼠线栓模型 改良手术方法 死亡原因 

分 类 号：R743.3[医药卫生—神经病学与精神病学]