DNA修饰碳纳米管电极对DNA与亚甲基蓝相互作用的研究  

DNA Modified Multi-walled Carbon Nanotubes Electrode for the Study of Interaction between DNA and Methylene Blue

作　　者：张怀[1] 张云怀[1] 李静[1] 肖鹏[2] 乔雷[1] 李泽全[1] 

机构地区：[1]重庆大学化学化工学院,重庆400030 [2]重庆大学数理学院,重庆400030

出　　处：《分析测试学报》2008年第10期1035-1038,共4页Journal of Instrumental Analysis

基　　金：重庆市自然科学基金资助项目(CSTS2005BB4200);重庆大学研究生科技创新基金资助项目(200701Y1A0140200)

摘　　要：通过羧基化的多壁碳纳米管（MWNTs）电极表面的-CO0H与DNA末端的-NH2，在N-羟基琥珀酰亚胺（NHS）和1-乙基一3-（3-二甲基氨丙基）碳二亚胺盐酸盐（EDC）活化作用下，制备了DNA共价修饰MWNTs电极。采用循环伏安法系统研究了崮定在MWNTs电极表面的DNA与亚甲基蓝（MB）之间的相互作用。实验结果表明：在20～200mV·s^-1范围内，MB在DNA修饰MWNTs电极上的氧化还原过程为表面控制过程；在pH5．1～7．6范围内，MB在DNA修饰MWNTs电极上的峰电位随pH的增加向负方向移动。A deoxyribonucleic acid （DNA） modified multi-walled carbon nanotubes （ MWNTs ） electrode was prepared by covalently binding earboxyl groups of the nanotubes and amino groups at the ends of DNA oligonucleotides through the activation of N-hydroxy-suceinimide （NHS） and 1-ethyl-3-（3-dimethylaminopropyl） carbodiimide hydroehloride （ EDC ）. The interactions of DNA immobilized on MWNTs electrode with methylene blue（MB） were investigated by cyclic vohammetry （CV）. The experimental results showed that the anodic and cathodic peak potentials were both shifted to negative direction with increasing pH from 5.1 to 7.6. The redox currents of MB at DNA modified MWNTs electrode had linear relationship under scan rate from 20 to 200 mV·s^-1, which indicated that the redox reaction was a surface controlled process.

关 键 词：多壁碳纳米管 脱氧核糖核酸(DNA) 修饰 亚甲基蓝 电化学 

分 类 号：O657.15[理学—分析化学] Q523[理学—化学]