应用SELDI技术检测反义寡核苷酸作用前后胃癌细胞培养液中的差异蛋白  

Detection of Different Protein in Culture Fluid of Gastric Carcinoma Cells Treated with Antisense Oligodeoxynucleotide as Revealed with SELDI Mass Spectrometry

作　　者：刘池波[1] 潘春琴[1] 蔡艳群[1] 

机构地区：[1]浙江省台州市立医院,318000

出　　处：《放射免疫学杂志》2008年第5期447-449,共3页Journal of Radioimmanology

摘　　要：目的:利用针对人端粒酶RNA(hTR)的反义寡核苷酸(ASODN)和正义寡核苷酸(NODN)作用于人胃癌细胞SGC7901,比较ASODN和NODN作用后细胞培养液蛋白的变化。方法:利用SELDI技术检测差异蛋白的表达变化。对照组为未加任何处理因素的SGC7901细胞。结果:SELDI技术检测发现,ASODN作用组有31个差异蛋白分子低表达,10个差异蛋白分子高表达。NODN作用组有25个差异蛋白分子低表达,16个差异蛋白分子高表达。所有差异蛋白分子量均<10000KDa。ASODN作用组中有6个低表达蛋白在NODN作用组高表达,分子量分别为4180.7KDa、4825KDa、7925.2KDa、8138KDa、8605.1KDa、8935.3KDa。结论:ASODN和NODN作用SGC7901细胞后细胞培养液中所表达的差异蛋白十分相似。Objective To compare the changes of protein expression in culture fluid of gastric carcinoma SGC7901 cells treated with antisense oligodeoxynucleotide （ASODN） and nonsense oligodeoxynucleotide （NODN） aimed at human telomerase RNA. Methods SELDI technology was used to detect the changes of protein expression after treatment with ASODN and NODN, the control was untreated. Results The results of SELDI showed that expressions of 31 proteins down regulated and 10 proteins up regulated in ASODN group, expressions of 28 proteins down regulated and 13 proteins up regulated in NODN group. The molecular weight of these different proteins were all less than 15000 Da. Expressions of six proteins （4180.7 KDa, 4825 KDa, 7925.2 KDa, 8138 KDa, 8605.1 KDa, 8935.3 KDa） were down regulated in ASODN group, but up regulated in NODN group. Conclusion The expressions of different proteins in ASODN group and in NODN group were quite similar.

关 键 词：寡核苷酸类 反义 胃癌 

分 类 号：R735.2[医药卫生—肿瘤] R454.1[医药卫生—临床医学]