Fas、FasL及Caspase-3在维甲酸诱导胃癌细胞凋亡中的作用  

Effect of Fas, FasL and Caspase-3 on apoptosis of retinoic acid-induced gastric carcinoma cells

作　　者：张红英[1] 李锦毅[1] 张建永[2] 

机构地区：[1]武警总医院,北京市100039 [2]辽宁医学院,辽宁省锦州市121001

出　　处：《世界华人消化杂志》2008年第29期3255-3260,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30672771~~

摘　　要：目的:探讨维甲酸诱导胃癌细胞BGC-803凋亡的作用及其与Fas、FasL、Caspase-3表达的关系.方法:以0.001、0.01、0.1、1、10、20μmol/L的维甲酸作用BGC-803细胞72h后,MTT法检测维甲酸对BGC-803细胞的生长抑制作用;流式细胞术分析维甲酸对胃癌BGC-803细胞凋亡的诱导作用;Hoechst33342/PI双荧光染色观察细胞凋亡;RT-PCR法检测Fas、FasL、Caspase-3基因的mRNA表达变化.结果:0.1、1、10、20μmol/L的维甲酸作用BGC-803细胞72h后,较对照组(未加药)能显著抑制细胞增殖(32.61%、44.42%、48.14%、51.15%vs0.657%,均P<0.01);20μmol/L维甲酸作用BGC-803细胞12、24和48h后,G2/M期细胞比例显著增加,出现凋亡特征性的亚G1峰;细胞出现染色质凝集、核膜破裂等凋亡特征;作用48h后,Fas、FasL、Caspase-3mRNA表达水平均较对照组显著上调.结论:Fas、FasL、Caspase-3参与了维甲酸诱导胃癌细胞凋亡的调控过程.,AIM： To explore the effect on apoptosis of retinoic acid （RA）-induced BGC-803 gastric carcinoma cells and its relationship with the expression of Fas, FasL and Caspase-3.METHODS： BGC-803 cells were treated with different concentrations of RA （0.001, 0.01, 0.1, 1, 10, 20 μmol/L） for 72 h. Then methyltetrazolium （MTT） assay was performed to determine the growth inhibition of BGC-803 cells; cell apoptosis rate was determined using flow cytometry; the feature of cell apoptosis was observed by Hoechst33342/PI staining; the mRNA expression of Fas, FasL and Caspase-3 were estimated using reverse transcription-polymerase chain reaction （RT-PCR）. RESULTS： After BGC-803 cells were treated with RA （0.1-20 μmol/L） for 72 h, RA inhibited the growth of cells significantly compared with that in the control group （32.61%, 44.42%, 48.14%, 51.15% vs 0.657%; all P 〈 0.01）. Cells in G2/M were significantly increased after the cells were treated with 20 μmol/L RA for 12 h, 24 h and 48 h. G1 peak specific to apoptosis was observed and also observed were chro- matic agglutination and rupture of caryon membrane. Expressions of Fas, FasL and Caspase-3 mRNA were up-regulated significantly by RA for 48 h compared with that in the control group. CONCLUSION： Fas, FasL and Caspase-3 are involved in gastric carcinoma cell apoptosis induced by RA.

关 键 词：FAS FASL CASPASE-3 维甲酸 胃癌 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]