小儿哮喘发病中CD23、IFN和IgE的作用及其意义  

THE ROLE OF CD23,INTERFERONγ AND SERUM IgE LEVELS IN CHILDREN WITH ASTHMA

作　　者：周云桂[1] 黄绍良[1] 李树浓[1] 麦贤弟[1] 朱昌国[1] 黄花荣[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院儿科

出　　处：《中国免疫学杂志》1997年第6期381-383,共3页Chinese Journal of Immunology

基　　金：广东省卫生厅资助

摘　　要：应用ＡＰＡＡＰ方法，Ｄｏｔ－ｂｌｏｔ地高辛标记核酸杂交技术及ＡＢＣ－ＥＬＩＳＡ法分别检测哮喘患儿淋巴细胞ＣＤ２３ｍＲＮＡ，膜ＣＤ２３分子表达和患儿血浆和淋巴细胞诱生ＩＦＮ－γ水平。结果，哮喘患儿ＣＤ２３ｍＲＮＡ表达增高，外周血ＰＢＭＣ和Ｂ细胞的ＣＤ２３表达均显著增加，ＩＦＮ－γ诱生水平明显低下，血清ＩｇＥ水平异常升高，Ｂ细胞ＣＤ２３分子表达与ＩｇＥ水平之间显著正相关，麻疹疫苗治疗后Ｂ细胞ＣＤ２３分子表达明显降低，而ＩＦＮ－γ诱生水平仍低下。atic children by using dotblot hybrization of mRNA with PiG labelled CD23 DNA probe,CD23+ cells by APAAP staining,the plasma levels of IFNγ and the PHA induced IFNγ levels of PBMC by ELISA.The results showed as follow:the high expression of CD23 mRNA in patients with asthma,expression of CD23 molecules on PBMC and Bcells(CD23+ cells)also increased.The PHA induced IFNγ levels of PBMC in asthma group were lower than that in normal control group,serum IgE levels of asthmatic children obviously elevated,there was positive correlation between CD23 molecules expression and serum IgE levels.After treatment,CD23 molecules of Bcells obviously decreased and PHA induced IFNγ still in lower levels in asthmatic patients.

关 键 词：哮喘 CD23 IFN 基因表达 儿童 

分 类 号：R725.622.5[医药卫生—儿科]