口腔白色念珠菌凋亡现象的初步研究  

Study on oral Candida albicans apoptosis in vitro

作　　者：杨丞喆[1] 宫玮玉[1] 卢境婷[1] 张杰铌[1] 朱秀娟[1] 亓庆国[1] 

机构地区：[1]山东大学口腔医学院,山东济南250012

出　　处：《上海口腔医学》2008年第5期492-495,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30400498);2007年度国家大学生创新计划资助项目~~

摘　　要：目的:通过流式细胞技术和透射电镜观察等方法,观察口腔白色念珠菌在弱酸诱导下出现的凋亡现象,为进一步研究白色念珠菌凋亡的分子生物学机制提供新途径。方法:从口腔念珠菌病患者口腔中分离出白色念珠菌菌株,在体外用乙酸进行凋亡诱导后,使用Annexin-V/FITC荧光染色和流式细胞技术,确定处于早期和晚期凋亡细胞的数目比例,在透射电镜下观察白色念珠菌细胞出现的凋亡表现。采用SPSS11.5软件包对数据进行χ2检验。结果:口腔白色念珠菌在乙酸诱导下出现典型的凋亡表现,不同浓度的乙酸,诱导真菌细胞凋亡的能力不同。结论:低等真核细胞白色念珠菌存在凋亡现象,对其分子生物学机制的深入研究,可能为开发抗真菌药物提供新的途径。PURPOSE： Candida albicans is one of the main opportunistic pathogen for human , the aim of this study is to investigate the phenomena of apoptosis in oral Candida albicans induced by acetic acid. METHODS： The Candida albicans of clinical strains were induced to apoptosis by using a weak acid acetic acid.The apoptosis was detected by flow cytometry and TEM. The data were processed for Chi-square test using SPSS11.5 software package. RESULTS： Oral Candida albicans had classic apoptosis when induced by proper concentration of acetic acid, and different concentrations of acetic acid had variable ability of inducing apoptosis of Candida albicans. CONCLUSIONS： Apoptosis can be detected in clinical strains of Candida albicans, the mechanism of apoptosis needs further research for the purpose of developing new antifungal drugs. Supported by National Natural Science Foundation of China（Grant No.30400498） and 2007 National College Student Innovative Planning Project.

关 键 词：口腔白色念珠菌 凋亡 乙酸 

分 类 号：R780.2[医药卫生—口腔医学]