流体剪切力对成骨细胞凋亡及bcl-2、Bax、Caspase-3蛋白表达的影响  

Effects of fluid shear stress on apoptosis of osteoblasts and expression of bcl-2,Bax and caspase-3

作　　者：刘劲松[1] 麻健丰[1] 王琪[2] 张大风[1] 刘传通[1] 梁星[2] 

机构地区：[1]温州医学院附属口腔医院,浙江温州325027 [2]四川大学华西口腔医学院修复科,四川成都610041

出　　处：《上海口腔医学》2008年第5期501-504,共4页Shanghai Journal of Stomatology

基　　金：浙江省教育委员会科研基金(20061823)~~

摘　　要：目的:探讨流体剪切力对成骨细胞凋亡及相关蛋白bcl-2、Bax、Caspase-3表达的影响。方法:对培养的第3代新生大鼠成骨细胞分别加载0(对照组)、0.6、1.0、2.0、3.0、4.0、5.0Pa流体剪切力1h后,静止培养24h。应用TUNEL法检测凋亡,采用免疫组化法检测bcl-2、Bax、Caspase-3蛋白。采用SPSS11.5软件包对数据进行单因素方差分析。结果:在0、0.6、1.0、2.0Pa组,细胞凋亡及bcl-2、Caspase-3表达量无显著差异,P>0.05。3.0、4.0、5.0Pa组间,随剪切力增大,细胞凋亡增加,Bcl-2表达量减少,Caspase-3增加,P<0.05。所有实验组间Bax无显著差异,P>0.05。结论:生理范围的流体剪切力对成骨细胞凋亡无影响,而过大力值导致bcl-2表达减少,进而活化caspase-3,成骨细胞凋亡增加。PURPOSE： The aim of the present investigation is to evaluate the effects of fluid shear stress on the cells of osteoblasts and to determine the mechanisms involved. METHODS： The fluid shear stress with a magnitude of 0（control group）, 0.6, 1.0, 2.0, 3.0, 4.0, 5.0 Pa was exerted on the third passage of osteoblasts for 1 hour,and incubation in static condition for 24 hours. The cell apoptotic index was tested by the terminal transferase dUTP nick end-labeling （TUNEL） method. The expression of bcl-2 ,Bax and caspase-3 were detected by immunohistochemical method. Statistical analysis was performed using SPSS11.5 software package for one-way ANOVA. RESULTS： The apoptosis of osteoblasts and the expression of bcl-2, Caspase-3 were not affected significantly by the fluid shear stress with a magnitude from 0 to 2.0 Pa （P〉0.05） . But in the 3.0,4.0 and 5.0 Pa groups, the number of apoptotic cells and the expression of caspase-3 increased while the expression of bcl-2 decreased （P〈0.05）.There was no significant difference in expression of Bax in all groups. CONCLUSIONS： The physiological fluid shear stress does not affect apoptosis of osteoblasts. However, the fluid shear stress of over-magnitude may lead to the decrease the expression of bcl-2,which results in activation of caspase-3 and apoptosis of osteoblasts. Supported by Research Grant from Zhejiang Provincial Education Committee（Grant No.20061823）.

关 键 词：成骨细胞:细胞凋亡:流体剪切力 

分 类 号：R78[医药卫生—口腔医学]