全冠修复材料对人牙龈成纤维细胞的毒性  

Cytotoxicity of several materials of full crowns on human gingival fibroblast cells in vitro

作　　者：马红[1] 刘梅[1] 章非敏[1] 王娟[1] 

机构地区：[1]南京医科大学口腔医学研究所,江苏南京210029

出　　处：《上海口腔医学》2008年第5期510-514,共5页Shanghai Journal of Stomatology

基　　金：江苏省"科教兴卫工程"医学重点人才项目(RC2007099)~~

摘　　要：目的:观察镍铬合金、金合金、铸造陶瓷对人牙龈成纤维细胞(human gingival fibroblast cells,HGFC)的毒性。方法:制备镍铬合金、金合金、铸造陶瓷的细胞培养基浸提液,采用四唑盐比色分析法(methyl thiazolyl tetrazolium assay,MTT法)测定材料对HGFC生长的影响;在材料周围接种HGFC,观察细胞的生长情况。采用Stata9.1软件对实验结果进行析因分析。结果:金合金的细胞相对增殖率为1.016、1.014、0.824、0.796,镍铬合金的细胞相对增殖率为1.028、1.079、0.903、0.809,铸造陶瓷的细胞相对增殖率为1.018、1.030、0.924、0.818。金合金和镍铬合金对细胞相对增殖率的影响有统计学差异(P=0.021),但金合金与铸造陶瓷、镍铬合金与铸造陶瓷之间无统计学差异(P>0.05)。金合金、镍铬合金和铸造陶瓷的细胞毒性级别(cytotoxical grade,CG)为0-1级。倒置显微镜观察3种材料周围均有细胞生长,镍铬合金周围有细胞坏死带产生。结论:镍铬合金与HGFC直接接触引起的细胞损伤比析出的金属离子对细胞损伤大。金合金和铸造陶瓷具有良好的生物相容性。PURPOSE： To evaluate the cytotoxicity of Ni-Cr base metal alloy, gold casting alloy and castable dental ceramic on human gingival fibroblast cells （HGFC） in vitro. METHODS： HGFC were cultured with extractions of Ni-Cr base metal alloy, gold casting alloy and castable dental ceramic. Relative growth rate （RGR） was analyzed with a methyl thiazolyl tetrazolium （MTT） assay. The shape of HGFCs was observed when HGFCs were directly contacted with three prosthodontic materials. Factor analysis was performed using Stata 9.1 software. RESULTS： The relative growth rates of HGFCs cultured with gold casting alloy were 1.016,1.014,0.824 and 0.796,respectively. The relative growth rates of HGFCs cultured with N^-C~ base metal alloy were 1.028,1.079,0.903 and 0.809,respectively. The relative growth rates of HGFCs cultured with castable dental ceramic were 1.018,1.030,0.924 and 0.818, respectively. There was significant difference of RGR between the gold casting alloy and the Ni-Cr base metal alloy groups（P=0.021）. No significant difference of RGR was observed between the gold casting alloy and castable dental ceramic groups （P〉0.05）.No significant difference of RGR was observed between the Ni-Cr base metal alloy and castable dental ceramic groups（P〉0.05）. Cytotoxical grades of Ni-Cr base metal alloy, gold casting alloy and castable dental ceramic were 0 to 1. HGFC could grow around the three prosthodontic materials. CONCLUSIONS： Ni-Cr base metal alloy has more cytotoxic effect in direct contact test than in extraction test on HGFC. Gold casting alloy and castable dental ceramic have good biocompatibility.Supported by Medical PI Project for Provincial Advancement through Science and Education of Jiangsu Province （RC2007099）.

关 键 词：人牙龈成纤维细胞 四唑盐比色分析法 镍铬合金 金合金 铸造陶瓷 

分 类 号：R783.1[医药卫生—口腔医学]