高频电刺激对PC12细胞形态及代谢水平的影响  

Effect of high-frequency electrical stimulation on morphology and metabolism of PC12 cells

作　　者：沃雁[1] 丁文龙[1] 夏蓉[1] 朱平[2] 朱浩[1] 王文进[1] 

机构地区：[1]上海交通大学医学院解剖学教研室,上海200025 [2]上海交通大学医学院细胞生物学教研室,上海200025

出　　处：《解剖学杂志》2008年第5期680-683,共4页Chinese Journal of Anatomy

基　　金：上海市教委第四期基础医学重点学科研究基金(2005JY03)

摘　　要：目的:研究细胞外高频电刺激(HFS)对PC12类交感神经细胞的形态及代谢水平的影响。方法:将HFS作用于传代的PC12类交感神经细胞株,高频电流参数为130 Hz,500μA,60μs;每天固定时间刺激3 h,持续3 d。分别用H-E染色、免疫组织化学、电子显微镜等方法检测经HFS后PC12类交感神经细胞在光镜、电镜下的形态变化及细胞代谢水平的变化。结果:相比对照组,HFS后PC12类神经细胞光镜下突起长度显著缩短,突起个数明显减少;酪氨酸羟化酶免疫组织化学结果显示HFS后PC12细胞胞质灰度降低,提示酪氨酸羟化酶代谢水平下降;电镜下可见胞质中的线粒体、分泌颗粒减少,突起和突触减少。结论:HFS对PC12类交感神经细胞有抑制作用,它抑制PC12类交感神经细胞突起的生长以及颗粒分泌的代谢水平。Objective： To explore effect of high-frequency electrical stimulation of extracellular （high frequency stimulation, HFS） on morphology and metabolism of sympathetic PC12 ceils. Methods： HFS was applied in the passage of the sympathetic PC12 cells under the current with parameters 130 Hz, 500 μA, 60μs, which were stimulated three hours each day for three days; H-E staining, immunohistochemistry and the electron transmission microscopy was used to detect the morphology and metabolism of the sympathetic PC12 cell after HFS under the light microscopy and the electron transmission microscopy. Results： Compared to the control group, the process length of the PC12 neural cells after HFS was significantly shortened and their number considerably reduced. Immunohistochemistry results showed that the gray of PC12 cell cytoplasm appeared shallowing after HFS, suggesting that tyrosine hydroxylase activity decreased; The numbers of visible mitochondria in the cytoplasm, secretory granules, processes and synapse were decreased. Conclusion： HFS inhibits the cellular propogation, the process growth and the metabolic secretion.

关 键 词：高频电刺激 肾上腺嗜铬细胞瘤细胞 形态 酪氨酸羟化酶 

分 类 号：R742.5[医药卫生—神经病学与精神病学]