GTL2基因在体细胞核移植牛中的印记状态分析  

Genomic Imprinting of GTL2 in Somatic Cell Nuclear Transfer Cattle

作　　者：王建春[1] 李冬杰[2] 杜卫华[3] 樊宝良[1] 李宁[4] 李世杰[1] 

机构地区：[1]河北农业大学生命科学学院,保定071001 [2]河北科技大学生物科学与工程学院,石家庄050018 [3]中国农科院北京畜牧兽医研究所,北京100094 [4]中国农业大学农业生物技术国家重点实验室,北京100094

出　　处：《生物化学与生物物理进展》2008年第10期1183-1187,共5页Progress In Biochemistry and Biophysics

基　　金：国家高技术研究发展计划(2007AA100504);河北省自然科学基金(C2006001032)资助项目~~

摘　　要：体细胞核移植技术(SCNT)在医学研究、畜牧业生产和拯救濒危动物方面有重要的应用价值,而核移植效率低是制约其应用的主要因素.印记基因在哺乳动物胚胎发育和出生后的正常生长中都具有十分重要的作用,GTL2基因是在人和鼠中已被鉴定的印记基因,它作为一种RNA调解分子调控目的mRNA的转录.为了研究GTL2基因在自然繁殖牛和体细胞核移植牛中的印记状态,首先应用PCR-SSCP方法对GTL2基因多态性进行检测,鉴定自然繁殖牛和体细胞核移植牛中的杂合子,进而利用RT-PCR-SSCP技术对GTL2基因在杂合子牛的心、肝、脾、肺、肾、大脑中的表达状态进行分析.研究结果表明:GTL2基因在自然繁殖牛的被检测的6个组织中均表现为单等位基因表达,在体细胞核移植牛的心和肝中表现为单等位基因表达,而在大脑、脾、肺、肾中为双等位基因表达,GTL2基因在体细胞核移植牛的部分组织中表达紊乱有可能是造成体细胞核移植牛器官发育异常和核移植效率低下的原因之一.Somatic cell nuclear transfer （SCNT） has great value in medicine, stock breeding and saving endangered animals, but the low efficiency of SCNT restricts its application. Imprinted genes regulate fetal growth and many are essential for normal development in mammals. GTL2 is imprinted in human and mouse, which act as noncoding RNAs regulating the translation of target mRNA. In order to identify the expression of GTL2 in cattle produced by natural reproduction and by SCNT, a single nucleotide polymorphisms （SNPs） in GTL2 through PCR-SSCP was identified and the GTL2 expression patterns in six organs of hybrids was analyzed by RT-PCR-SSCP. The results demonstrated that GTL2 was monoallelic expression in all six examined organs of cattle produced by natural reproduction, and showed monoallelic expression in heart and liver but biallelic expression in brain, spleen, lung and kidney of cattle produced by SCNT that died shortly after birth. The abnormal expression of GTL2 may contribute to the organ development defects and the low efficiency of SCNT.

关 键 词：牛 GTL2 基因组印记 体细胞核移植 

分 类 号：Q953[生物学—动物学] S814.8[农业科学—畜牧学]