Cx43对针灸治疗原发性痛经大鼠效应的影响  

Influence of Cx43 on acupuncture effect on the primary dysmenorrheal rat

作　　者：刘芳[1] 郑翠红[1] 黄光英[1] 王伟[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院神经内科,湖北武汉430030

出　　处：《中国针灸》2008年第10期751-756,共6页Chinese Acupuncture & Moxibustion

基　　金：国家重点基础研究计划(973计划)资助项目:2006CB504502;国家自然科学基金项目:90209009

摘　　要：目的:观察沉默穴位局部缝隙连接蛋白(connexin43,Cx43)的表达对针刺效应的影响,探讨针刺治疗原发性痛经的机制。方法:以催产素造成大鼠原发性痛经模型,应用RNA干扰(RNAi)技术沉默穴位局部Cx43的表达。将50只SD雌性大鼠分为正常组(N)、模型组(M)、针刺组(A)、针刺+干扰组(A+I)、针刺+干扰对照组(A+IC)。应用RT-PCR方法检测大鼠子宫的催产素受体(oxytocin receptor,OTR)、加压素受体(vasopressin receptor,VPR)的mRNA表达情况;放免法和ELISA检测血浆中前列腺素E2(PGE2)和PGF2α的含量。结果:①A组和A+IC组的大鼠扭体次数(9.43±3.87,10.28±4.23)明显低于M组和A+I组(15.43±5.13,17.00±3.87),潜伏期(12.43±3.46,11.00±3.65)min高于M组(7.57±1.99)min和A+I组(8.43±2.57)min(P<0.05)。②A+I组穴位处的Cx43 mRNA和蛋白表达低于N组(P<0.05)。③A组和A+IC组的OTR、VPR的mRNA表达较M组和A+I组降低,且差异有统计学意义(均P<0.05);M组和A+I组差异无统计学意义(P>0.05)。④A组和A+IC组的PGE2升高,PGF2α降低,较M组差异有统计学意义(P<0.05)。结论:沉默穴位局部Cx43能有效抑制针刺效应,通过降低痛经大鼠子宫内膜OTR、VPR及调节前列腺素合成系统,可能是针刺治疗原发性痛经的作用机制之一。Objective To observe the influence of silencing Connexin43 （Cx43） expression of partial acupoints on acupuncture effect, so as to probe into the mechanism of acupuncture treatment for primary dysmenorrhea. Methods The primary dysmenorrheal rat model made by oxytocin and RNA interference （RNAi） technology was used to silence the expression of Cx43 in acupoints. Fifty SD female rats were divided into five groups, a normal group （N）, a model group （M）, an acupuncture group （A）, an acupuncture plus interference group （A＋I）, an acupuncture plus interference control group （A＋IC）. RT-PCR method was used to observe the oxytocin receptor （OTR） and vasopressin receptor （VPR） mRNA expressions in the uterus in each group. Plasma prostaglandin E2 （PGE2） and PGF2α levels were detected by radioimmunoassay and ELISA, respectively. Results （1） The times of writhing body （9.43±3.87 and 10.28±4.23） were significantly lower and the latency period of writhing body （12.43±3.46, 11.00±3.65） were longer in the group A and the group A＋IC as compared with （15.43±5.13, 17.00±3.87） and （7.57±1.99, 8.43±2.57） in the group M and group A＋I （P〈0.05）, respectively. （2） The levels of Cx43 mRNA level and protein expression of acupoint in the group A＋I were significantly lower than those of the group N （P〈0.05）. （3） OTR and VPR mRNA in the uterus in the group A and the group A＋IC were significantly lower than those in the group M and the group A＋I （P〈0.05）, with no significant difference between the group M and the group A＋I （P〉0.05）. （4） As compared with the group M, PGE2 level increased and PGF2α level decreased in the group A and the group A＋IC （P〈0.05）. Conclusion Silencing Cx43 expression of partial acupoint can inhibit effectively the effect of acupuncture through decreasing OTR and VPR in endometrium of the dysmenorrheal rat and adjusting the prostaglandins （PGs） synthesis system, which possibly is one of the

关 键 词：原发性痛经 大鼠 Sprague-Dawley 连接蛋白43 RNA 信使 针刺镇痛原理 

分 类 号：R224.1[医药卫生—中医基础理论]